Human tryptase beta 2 ELISA Kit | TPSB2 elisa kit

Human Tryptase beta-2, TPSB2 ELISA Kit

Cat. Num. AAA923703

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Human
• Synonyms: tryptase beta 2; Human Tryptase beta-2; TPSB2 ELISA Kit; Human Tryptase beta-2 (TPSB2) ELISA kit; TPS2; tryptaseB; tryptaseC; OTTHUMP00000208944; mast cell tryptase beta II; mast cell tryptase beta III; tryptase II; tryptase III; tryptase beta II; tryptase beta III; TPSB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TPM4. No significant cross-reactivity or interference between human TPM4 and analogues was observed.
• Sequence Length: 275

• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: 5.8 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPSB2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPM4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 13775595
• NCBI GeneID: 7177
• NCBI Accession #: NP_003285.2
• NCBI GenBank Nucleotide #: NM_003294.3
• UniProt Accession #: P20231; O95827; Q15664; Q9UQI6; Q9UQI7; D2E6S0; D2E6S2; Q15661
• Molecular Weight: 30,515 Da
• NCBI Official Full Name: tryptase alpha/beta-1
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase
• UniProt Protein Name: Tryptase beta-2
• Protein Family: Tryptase
• UniProt Gene Name: TPSB2
• UniProt Synonym Gene Names: TPS2; Tryptase-2
• UniProt Entry Name: TRYB2_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily.

Protein type: EC 3.4.21.59; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis