Rat tryptase alpha/beta 1 ELISA Kit | TPSAB1 elisa kit

Rat Mast Cell Tryptase, MCT ELISA Kit

Cat. Num. AAA701634

• Gene Names: Tpsab1; Mcpt7; TPSB1; rMCP-7
• Reactivity: Rat
• Synonyms: tryptase alpha/beta 1; Rat Mast Cell Tryptase; MCT ELISA Kit; TPS1; TPS2; TPSB1; mast cell alpha II tryptase; mast cell beta I tryptase; tryptase alpha II; tryptase beta 1; tryptase beta I; tryptase-I; tryptase-III; TPSAB1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat MCT. No significant cross-reactivity or interference between rat MCT and analogues was observed.
• Sequence Length: 310

• Samples: Serum, plasma, urine
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625 ng/ml-40 ng/ml
• Sensitivity: < 0.156 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPSAB1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 194239635
• NCBI GeneID: 54271
• NCBI Accession #: NP_062195.2
• NCBI GenBank Nucleotide #: NM_019322.2
• UniProt Accession #: P27435; P27436
• Molecular Weight: 30,400 Da
• NCBI Official Full Name: tryptase
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: Tpsab1
• NCBI Official Synonym Symbols: Mcpt7; TPSB1; rMCP-7
• NCBI Protein Information: tryptase; MMCP-7; tryptase, skin; tryptase beta 1; mast cell protease 7; tryptase alpha/beta-1
• UniProt Protein Name: Tryptase
• Protein Family: Tryptase
• UniProt Gene Name: Tpsab1
• UniProt Synonym Gene Names: Mcp7; Mcpt7; Tpsb1; rMCP-7
• UniProt Entry Name: TRYB1_RAT

NCBI Description

serine protease in mast cells [RGD, Feb 2006]

Uniprot Description

Function: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.

Catalytic activity: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.