Thyroid peroxidase Recombinant Protein | TPO recombinant protein

Recombinant Human Thyroid peroxidase

Cat. Num. AAA959768

• Gene Names: TPO; MSA; TPX; TDH2A
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Thyroid peroxidase; Recombinant Human Thyroid peroxidase; TPO recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 19-161. Partial
• Sequence: FFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSPAQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPPKCPNTCLANKYRPITGACNNR

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for TPO recombinant protein

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Product Categories/Family for TPO recombinant protein

Cancer

References

Human thyroid peroxidase complete cDNA and protein sequence, chromosome mapping, and identification of two alternately spliced mRNAs.Kimura S., Kotani T., McBride O.W., Umeki K., Hirai K., Nakayama T., Ohtaki S.Proc. Natl. Acad. Sci. U.S.A. 84:5555-5559(1987) Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA.Libert F., Ruel J., Ludgate M., Swillens S., Alexander N., Vassart G., Dinsart C.Nucleic Acids Res. 15:6735-6735(1987) Structure of the human thyroid peroxidase gene comparison and relationship to the human myeloperoxidase gene.Kimura S., Hong Y.S., Kotani T., Ohtaki S., Kikkawa F.Biochemistry 28:4481-4489(1989) Nucleotide sequence of the alternatively spliced human thyroid peroxidase cDNA, TPO-2.Barnett P.S., Banga J.P., Watkins J., Huang G.C., Gluckman D.R.B., Page M.J., McGregor A.M.Nucleic Acids Res. 18:670-670(1990) Rapoport B. Homo sapiens thyroid peroxidase (TPO) variant mRNA, alternatively spliced sequence.Hennen G.P., Igout A., Melen L.B. Increasing diversity of human thyroperoxidase generated by alternative splicing. Characterization by molecular cloning of new transcripts with single- and multispliced mRNAs.Ferrand M., Le Fourn V., Franc J.-L.J. Biol. Chem. 278:3793-3800(2003) Isolation of a complementary DNA clone for thyroid microsomal antigen. Homology with the gene for thyroid peroxidase.Seto P., Hirayu H., Magnusson R.P., Gestautas J., Portmann L., Degroot L.J., Rapoport B.J. Clin. Invest. 80:1205-1208(1987) Evidence for an alternate splicing in the thyroperoxidase messenger from patients with Graves' disease.Zanelli E., Henry M., Charvet B., Malthiery Y.Biochem. Biophys. Res. Commun. 170:735-741(1990) Identification of a novel partner of duox EFP1, a thioredoxin-related protein.Wang D., De Deken X., Milenkovic M., Song Y., Pirson I., Dumont J.E., Miot F.J. Biol. Chem. 280:3096-3103(2005) Identification of five novel inactivating mutations in the human thyroid peroxidase gene by denaturing gradient gel electrophoresis.Bikker H., Vulsma T., Baas F., de Vijlder J.J.M.Hum. Mutat. 6:9-16(1995) Molecular analysis of mutated thyroid peroxidase detected in patients with total iodide organification defects.Bikker H., Baas F., De Vijlder J.J.M.J. Clin. Endocrinol. Metab. 82:649-653(1997) A novel mutation in the TPO gene in goitrous hypothyroid patients with iodide organification defect.Santos C.L.S., Bikker H., Rego K.G.M., Nascimento A.C., Tambascia M., De Vijlder J.J.M., Medeiros-Neto G.Clin. Endocrinol. (Oxf.) 51:165-172(1999) Two different mutations in the thyroid peroxidase gene of a large inbred Amish kindred power and limits of homozygosity mapping.Pannain S., Weiss R.E., Jackson C.E., Dian D., Beck J.C., Sheffield V.C., Cox N., Refetoff S.J. Clin. Endocrinol. Metab. 84:1061-1071(1999) A novel mutation in the human thyroid peroxidase gene resulting in a total iodide organification defect.Kotani T., Umeki K., Yamamoto I., Maesaka H., Tachibana K., Ohtaki S.J. Endocrinol. 160:267-273(1999) Two decades of screening for congenital hypothyroidism in The Netherlands TPO gene mutations in total iodide organification defects (an update) .Bakker B., Bikker H., Vulsma T., de Randamie J.S.E., Wiedijk B.M., De Vijlder J.J.M.J. Clin. Endocrinol. Metab. 85:3708-3712(2000) Novel mutations of the thyroid peroxidase gene in patients with permanent congenital hypothyroidism.Ambrugger P., Stoeva I., Biebermann H., Torresani T., Leitner C., Grueters A.Eur. J. Endocrinol. 145:19-24(2001) Two novel missense mutations in the thyroid peroxidase gene, R665W and G771R, result in a localization defect and cause congenital hypothyroidism.Umeki K., Kotani T., Kawano J., Suganuma T., Yamamoto I., Aratake Y., Furujo M., Ichiba Y.Eur. J. Endocrinol. 146:491-498(2002) High prevalence of a novel mutation (2268 insT) of the thyroid peroxidase gene in Taiwanese patients with total iodide organification defect, and evidence for a founder effect.Niu D.-M., Hwang B., Chu Y.-K., Liao C.-J., Wang P.-L., Lin C.-Y.J. Clin. Endocrinol. Metab. 87:4208-4212(2002) Mutation analysis of thyroid peroxidase gene in Chinese patients with total iodide organification defect identification of five novel mutations.Wu J.-Y., Shu S.-G., Yang C.-F., Lee C.-C., Tsai F.-J.J. Endocrinol. 172:627-635(2002) Genetics of specific phenotypes of congenital hypothyroidism a population-based approach.Calaciura F., Miscio G., Coco A., Leonardi D., Cisternino C., Regalbuto C., Bozzali M., Maiorana R., Ranieri A., Carta A., Buscema M., Trischitta V., Sava L., Tassi V.Thyroid 12:945-951(2002) Partial iodide organification defect caused by a novel mutation of the thyroid peroxidase gene in three siblings.Kotani T., Umeki K., Kawano J., Suganuma T., Hishinuma A., Ieiri T., Harada S.Clin. Endocrinol. (Oxf.) 59:198-206(2003) Five novel inactivating mutations in the thyroid peroxidase gene responsible for congenital goiter and iodide organification defect.Rivolta C.M., Esperante S.A., Gruneiro-Papendieck L., Chiesa A., Moya C.M., Domene S., Varela V., Targovnik H.M.Hum. Mutat. 22:259-259(2003) Monoallelic expression of mutant thyroid peroxidase allele causing total iodide organification defect.Fugazzola L., Cerutti N., Mannavola D., Vannucchi G., Fallini C., Persani L., Beck-Peccoz P.J. Clin. Endocrinol. Metab. 88:3264-3271(2003) Two novel mutations in the thyroid peroxidase gene with goitrous hypothyroidism.Tajima T., Tsubaki J., Fujieda K.Endocr. J. 52:643-645(2005) Goitrous congenital hypothyroidism and hearing impairment associated with mutations in the TPO and SLC26A4/PDS genes.Pfarr N., Borck G., Turk A., Napiontek U., Keilmann A., Mueller-Forell W., Kopp P., Pohlenz J.J. Clin. Endocrinol. Metab. 91:2678-2681(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 28558982
• NCBI GeneID: 7173
• NCBI Accession #: NP_000538.3
• NCBI GenBank Nucleotide #: NM_000547.5
• UniProt Accession #: P07202; P09934; P09935; Q8IUL0; Q8NF94; Q8NF95; Q8NF96; Q8NF97; Q8TCI9
• Molecular Weight: 17.9 kDa
• NCBI Official Full Name: thyroid peroxidase isoform a
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: TPO
• NCBI Official Synonym Symbols: MSA; TPX; TDH2A
• NCBI Protein Information: thyroid peroxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: TPO
• UniProt Synonym Gene Names: TPO
• UniProt Entry Name: PERT_HUMAN

NCBI Description

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

Uniprot Description

TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Oxidoreductase; EC 1.11.1.8

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: cell surface; extracellular space; integral to plasma membrane; mitochondrion; plasma membrane

Molecular Function: calcium ion binding; heme binding; iodide peroxidase activity; peroxidase activity

Biological Process: embryonic hemopoiesis; hormone biosynthetic process; hydrogen peroxide catabolic process; response to lipid; thyroid hormone generation

Disease: Thyroid Dyshormonogenesis 2a