Typical Testing Data/Standard Curve (for reference only)
Human Solute Carrier Family 1, Member 5 (SLC1A5) ELISA Kit | SLC1A5 elisa kit
Human Solute Carrier Family 1, Member 5 (SLC1A5) ELISA Kit
Gene Names
SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
Reactivity
Human
Synonyms
Solute Carrier Family 1; Member 5 (SLC1A5); Human Solute Carrier Family 1; Member 5 (SLC1A5) ELISA Kit; SLC1A5; AAAT; ASCT2; ATBO; M7V1; M7VS1; R16; RDRC; Neutral Amino Acid Transporter B(0); Baboon M7 virus receptor; Sodium-dependent neutral amino acid transporter type 2; SLC1A5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SLC1A5. No significant cross-reactivity or interference between SLC1A5 and analogues was observed.
Sequence Length
313
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.112ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLC1A5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLC1A5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SLC1A5 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SLC1A5 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC1A5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLC1A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLC1A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SLC1A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC1A5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLC1A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLC1A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SLC1A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,710 Da
NCBI Official Full Name
neutral amino acid transporter B(0) isoform 2
NCBI Official Synonym Full Names
solute carrier family 1 member 5
NCBI Official Symbol
SLC1A5
NCBI Official Synonym Symbols
R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
NCBI Protein Information
neutral amino acid transporter B(0)
UniProt Protein Name
Neutral amino acid transporter B(0)
Protein Family
UniProt Gene Name
SLC1A5
UniProt Synonym Gene Names
ASCT2; M7V1; RDR; RDRC; ATB(0)
NCBI Description
The SLC1A5 gene encodes a sodium-dependent neutral amino acid transporter that can act as a receptor for RD114/type D retrovirus (Larriba et al., 2001 [PubMed 11781704]).[supplied by OMIM, Jan 2011]
Uniprot Description
Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids (PubMed:8702519). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904).
Research Articles on SLC1A5
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Product Notes
The Human SLC1A5 slc1a5 (Catalog #AAA451053) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA451053 ELISA Kit recognizes Human SLC1A5. It is sometimes possible for the material contained within the vial of "Solute Carrier Family 1, Member 5 (SLC1A5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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