Human Tenascin-R ELISA Kit | TNR elisa kit

Human Tenascin-R PicoKine ELISA Kit

Cat. Num. AAA1752604

• Gene Names: TNR; TN-R
• Reactivity: Human
• Synonyms: Tenascin-R; Human Tenascin-R PicoKine ELISA Kit; Human Tenascin-R; tenascin R; TN-R; Janusin; Restrictin; TNR; TNR elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TNR
• Sequence Length: 1358

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNR elisa kit

Principle of the Assay: The Picokine™ Human TNR Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNR with a 96-well strip plate that is pre-coated with antibody specific for TNR. The detection antibody is a biotinylated antibody specific for TNR. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human TNR with immunogen: Expression system for standard: NSO, Immunogen sequence: Q32-F1358. The kit is analytically validated with ready to use reagents. To measure Human TNR, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human TNR in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNR in the sample.!!Background/Introduction: Tenascin-R is a protein that in humans is encoded by the TNR gene. This gene is mapped to 1q25.1. Tenascin-R (TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion), TNX (TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The encoded protein is restricted to the central nervous system. The protein may play a role in neurite outgrowth, neural cell adhesion and modulation of sodium channel function. It is a constituent of perineuronal nets.

NCBI and Uniprot Product Information

• NCBI GI #: 157384973
• NCBI GeneID: 7143
• NCBI Accession #: NP_003276.3
• NCBI GenBank Nucleotide #: NP_003276.3
• UniProt Accession #: Q92752
• NCBI Official Full Name: tenascin-R isoform 1
• NCBI Official Synonym Full Names: tenascin R
• NCBI Official Symbol: TNR
• NCBI Official Synonym Symbols: TN-R
• NCBI Protein Information: tenascin-R
• UniProt Protein Name: Tenascin-R
• Protein Family: Tenascin
• UniProt Gene Name: TNR
• UniProt Synonym Gene Names: TN-R
• UniProt Entry Name: TENR_HUMAN

NCBI Description

This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The encoded protein is restricted to the central nervous system. The protein may play a role in neurite outgrowth, neural cell adhesion and modulation of sodium channel function. It is a constituent of perineuronal nets. [provided by RefSeq, Aug 2013]

Uniprot Description

TNR: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. Belongs to the tenascin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region; nucleus; lipid raft

Biological Process: negative regulation of axon extension involved in regeneration; axon guidance; extracellular matrix organization and biogenesis; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission; synapse organization and biogenesis; neuron adhesion; negative regulation of cell-cell adhesion; cell adhesion; neuromuscular process controlling balance; associative learning; positive regulation of transmission of nerve impulse; telencephalon cell migration