Sheep Tenascin R ELISA Kit | TNR elisa kit

Sheep Tenascin R ELISA Kit

Cat. Num. AAA734240

• Gene Names: TNR; TN-R
• Reactivity: Sheep
• Synonyms: Tenascin R; Sheep Tenascin R ELISA Kit; TNR elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This TNR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep TNR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNR elisa kit

Principle of the assay: TNR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TNR antibody and an TNR-HRP conjugate. The assay sample and buffer are incubated together with TNR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNR concentration since TNR from samples and TNR-HRP conjugate compete for the anti-TNR antibody binding site. Since the number of sites is limited, as more sites are occupied by TNR from the sample, fewer sites are left to bind TNR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TNR elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 157384973
• NCBI GeneID: 7143
• NCBI Accession #: NP_003276.3
• NCBI GenBank Nucleotide #: NM_003285.2
• Molecular Weight: 139,695 Da
• NCBI Official Full Name: tenascin-R
• NCBI Official Synonym Full Names: tenascin R
• NCBI Official Symbol: TNR
• NCBI Official Synonym Symbols: TN-R
• NCBI Protein Information: tenascin-R; janusin; restrictin
• UniProt Protein Name: Tenascin-R
• Protein Family: Tenascin
• UniProt Gene Name: TNR
• UniProt Synonym Gene Names: TN-R
• UniProt Entry Name: TENR_HUMAN

NCBI Description

This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The encoded protein is restricted to the central nervous system. The protein may play a role in neurite outgrowth, neural cell adhesion and modulation of sodium channel function. It is a constituent of perineuronal nets. [provided by RefSeq, Aug 2013]

Uniprot Description

TNR: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. Belongs to the tenascin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region; nucleus; lipid raft

Biological Process: negative regulation of axon extension involved in regeneration; axon guidance; extracellular matrix organization and biogenesis; synapse organization and biogenesis; neuron adhesion; negative regulation of cell-cell adhesion; neuromuscular process controlling balance; cell adhesion; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission; associative learning; positive regulation of transmission of nerve impulse; telencephalon cell migration