Mouse anti-Human Cardiac Troponin I Monoclonal Antibody | anti-TNNI3 antibody

Cardiac Troponin I Monoclonal Antibody

Cat. Num. AAA9415056

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein G Purifed
• Synonyms: Cardiac Troponin I; Monoclonal Antibody; Cardiac Troponin I Monoclonal Antibody; CTnI; anti-TNNI3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: Reacts equally with free cardiac troponin I and cardiac troponin I forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. No cross-reactivity with skeletal muscle troponin I.
• Purity/Purification: Protein G Purifed
• Form/Format: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 210

• Applicable Applications for anti-TNNI3 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:500 - 1:1000
• Immunogen: Recombinant Human Cardiac Troponin I protein
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(All lanes: Mouse monoclonal to Human Cardiac Troponin I at 1ug/ml)

Related Product Information for anti-TNNI3 antibody

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Product Categories/Family for anti-TNNI3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I3, cardiac type
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytosol; sarcomere; troponin complex

Molecular Function: actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding

Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; muscle filament sliding; negative regulation of ATPase activity; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Dilated, 2a; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Familial Restrictive, 1