Western Blot (WB)
(Anti-GCLC antibody, MBS175561, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Heart Tissue LysateLane 4: Rat Lung Tissue Lysate )
Rabbit GCLC Polyclonal Antibody | anti-GCLC antibody
Anti-GCLC antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Rat
Concluded Species: Hu, Ms
Antigen Retrieval: n/a
IHC-P:Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Concluded Species: Ms
Antigen Retrieval: By Heat
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Western Blot (WB)
(Anti-GCLC antibody, MBS175561, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Heart Tissue LysateLane 4: Rat Lung Tissue Lysate )
Western Blot (WB)
(Anti-GCLC antibody, MBS175561, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Heart Tissue LysateLane 4: Rat Lung Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-GCLC antibody, MBS175561, IHC(P)IHC(P): Human Rectal Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-GCLC antibody, MBS175561, IHC(P)IHC(P): Human Rectal Cancer Tissue )
Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
NCBI and Uniprot Product Information
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
Uniprot Description
GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.
Protein type: Ligase; Other Amino Acids Metabolism - glutathione; EC 6.3.2.2
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: glutamate-cysteine ligase complex; cytosol
Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; coenzyme binding; ADP binding; ATP binding
Biological Process: glutamate metabolic process; response to arsenic; L-ascorbic acid metabolic process; response to hormone stimulus; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; sulfur amino acid metabolic process; response to heat; cell redox homeostasis; xenobiotic metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of transcription, DNA-dependent; regulation of blood vessel size; regulation of mitochondrial depolarization; negative regulation of apoptosis
Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To
Research Articles on GCLC
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Product Notes
The GCLC gclc (Catalog #AAA175561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GCLC antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCLC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: Concentration: 0.1-0.5ug/ml Tested Species: Rat Concluded Species: Hu, Ms Antigen Retrieval: IHC-P:Concentration: 0.5-1ug/ml Tested Species: Hu, Rat Concluded Species: Ms Antigen Retrieval: By Heat Other applications have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the GCLC gclc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCLC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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