OX40L/TNFSF4 Recombinant Protein | TNFSF4 recombinant protein

OX40L/TNFSF4 Protein, Mouse, Recombinant (mFc Tag)

Cat. Num. AAA8123246

• Gene Names: Tnfsf4; Ath1; gp34; Ath-1; Ox40l; TXGP1; CD134L; OX-40L; Txgp1l
• Purity: >95% as determined by SDS-PAGE
• Synonyms: OX40L/TNFSF4; OX40L/TNFSF4 Protein; Mouse; Recombinant (mFc Tag); Mouse OX-40L/TNFSF4/CD252 Protein (mFc Tag); Ath-1 Protein; Ath1 Protein; CD134L Protein; gp34 Protein; OX-40L Protein; Ox40l Protein; TXGP1 Protein; Txgp1l Protein; tumor necrosis factor (ligand) superfamily; member 4; TNFSF4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln49-Leu198

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Tnfsf4 (NP_033478.1)(Gln49-Leu198) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for TNFSF4 recombinant protein

Background: OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Product Categories/Family for TNFSF4 recombinant protein

Cancer Drug Targets

References

Lei W. et al., 2012, Ann Acad Med Singapore. 41 (5): 200-4. Lee YH. et al., 2012, Hum Immunol. 73 (10): 1050-4. Weiguang Y. et al., 2012, PLoS One. 7 (8): e41277.

NCBI and Uniprot Product Information

• NCBI GI #: 6678473
• NCBI GeneID: 22164
• NCBI Accession #: NP_033478.1
• NCBI GenBank Nucleotide #: NM_009452.2
• UniProt Accession #: P43488
• Molecular Weight: 22,255 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 4
• NCBI Official Symbol: Tnfsf4
• NCBI Official Synonym Symbols: Ath1; gp34; Ath-1; Ox40l; TXGP1; CD134L; OX-40L; Txgp1l
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4; OX40 ligand; atherosclerosis 1; tax-transcriptionally activated glycoprotein 1 ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf4
• UniProt Synonym Gene Names: Ox40l; Txgp1l; OX40L
• UniProt Entry Name: TNFL4_MOUSE

Uniprot Description

TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.

Protein type: Cell cycle regulation; Membrane protein, integral; Cytokine

Cellular Component: extracellular space; cell surface; membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: negative regulation of T-helper 1 cell differentiation; positive regulation of interleukin-12 production; negative regulation of cytokine secretion; positive regulation of T cell cytokine production; positive regulation of activated T cell proliferation; defense response to nematode; positive regulation of interleukin-10 production; positive regulation of interleukin-4 production; T cell proliferation; positive regulation of immune effector process; regulation of adaptive immune response; positive regulation of T-helper 2 cell differentiation; negative regulation of regulatory T cell differentiation; negative regulation of interferon-gamma production; positive regulation of interleukin-13 production; inflammatory response; positive regulation of memory T cell differentiation; blood vessel development; cholesterol metabolic process; positive regulation of T-helper 2 type immune response; negative regulation of transcription factor activity; response to virus; positive regulation of immunoglobulin mediated immune response; cytokine production; positive regulation of interleukin-6 production; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of interleukin-2 production; innate immune response; immune response; positive regulation of alpha-beta T cell proliferation; negative regulation of transcription, DNA-dependent; acute inflammatory response; positive regulation of inflammatory response