OX40L/TNFSF4 Recombinant Protein | TNFSF4 recombinant protein

OX40L/TNFSF4 Protein, Human, Recombinant (ECD, mFc Tag)

Cat. Num. AAA8118681

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Purity: >90 % as determined by SDS-PAGE.
• Synonyms: OX40L/TNFSF4; OX40L/TNFSF4 Protein; Human; Recombinant (ECD; mFc Tag); Human OX-40L/TNFSF4/CD252 Protein (Fc Tag); tumor necrosis factor (ligand) superfamily; member 4; CD134L Protein; CD252 Protein; GP34 Protein; OX-40L Protein; OX4OL Protein; TXGP1 Protein; TNFSF4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 369aa
• Sequence: Gln51-Leu183

• Species: Human
• Tag: mFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Asp
• Protein Construction: |A DNA sequence encoding the human TNFSF4 (NP_003317.1) (Gln51-Leu183) was expressed with the Fc region of mouse IgG1 at the N-terminus
• Activity: Measured by its binding ability in a functional ELISA.Immobilized recombinant Human TNFRSF4-His (Cat:10481-H08H) at 10ug/ml (100ul/well) can bind human S4-mFc3-TNFSF4 (Cat:13127-H04H)with a linear range of 1.28-20ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized recombinant Human TNFRSF4-His (Cat:10481-H08H) at 10 ug/ml (100 ul/well) can bind human S4-mFc3-TNFSF4 (Cat:13127-H04H)with a linear range of 1.28-20 ug/ml.)

Related Product Information for TNFSF4 recombinant protein

OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig-and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.

Product Categories/Family for TNFSF4 recombinant protein

Cancer Drug Targets

References

Lei W. et al., 2012, Ann Acad Med Singapore. 41 (5): 200-4. Lee YH. et al., 2012, Hum Immunol. 73 (10): 1050-4. Weiguang Y. et al., 2012, PLoS One. 7 (8): e41277.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317.1
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q8IV74; Q9HCN9
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L
• UniProt Entry Name: TNFL4_HUMAN

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: extracellular space; cell surface; integral to plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: negative regulation of T-helper 1 cell differentiation; positive regulation of interleukin-12 production; negative regulation of cytokine secretion; positive regulation of T cell cytokine production; defense response to nematode; positive regulation of activated T cell proliferation; signal transduction; positive regulation of interleukin-10 production; positive regulation of interleukin-4 production; T cell proliferation; positive regulation of T-helper 2 cell differentiation; regulation of adaptive immune response; negative regulation of regulatory T cell differentiation; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; positive regulation of memory T cell differentiation; cholesterol metabolic process; positive regulation of T-helper 2 type immune response; response to virus; negative regulation of transcription factor activity; positive regulation of immunoglobulin mediated immune response; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of interleukin-2 production; positive regulation of alpha-beta T cell proliferation; immune response; negative regulation of transcription, DNA-dependent; acute inflammatory response; positive regulation of inflammatory response

Disease: Myocardial Infarction, Susceptibility To