Human tumor necrosis factor (ligand) superfamily, member 4 ELISA Kit | TNFSF4 elisa kit

Human soluble OX40L, sOX40L ELISA Kit

Cat. Num. AAA703272

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
• Reactivity: Human
• Synonyms: tumor necrosis factor (ligand) superfamily; member 4; Human soluble OX40L; sOX40L ELISA Kit; Human soluble OX40L (sOX40L) ELISA kit; CD134L; CD252; GP34; OX-40L; OX4OL; TXGP1; CD134 ligand; OX40 antigen ligand; tax-transcriptionally activated glycoprotein 1 (34kD) ; TNFSF4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human sOX40L. No significant cross-reactivity or interference between human sOX40L and analogues was observed.
• Sequence Length: 183

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml.
• Sensitivity: The minimum detectable dose of human sOX40Lis typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays):CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF4 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sOX40L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sOX40L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sOX40L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sOX40L bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317.1
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q9HCN9
• Molecular Weight: 21,050 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4; CD134 ligand; glycoprotein Gp34; OX40 antigen ligand; tax-transcriptionally activated glycoprotein 1 (34kD)
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L
• UniProt Entry Name: TNFL4_HUMAN

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Function: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.