Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit | TNFSF4 elisa kit

Human Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit

Cat. Num. AAA2702595

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Reactivity: Human
• Synonyms: Tumor Necrosis Factor Ligand Superfamily; Member 4 (TNFSF4); Human Tumor Necrosis Factor Ligand Superfamily; Member 4 (TNFSF4) ELISA Kit; CD252; TXGP1; OX40L; Gp34; OX40-L; Tax-Transcriptionally Activated Glycoprotein 1; 34kD; Glycoprotein Gp34; OX40 ligand; TNFSF4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4).; No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) and analogues was observed.
• Sequence Length: 183

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317.1
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q8IV74; Q9HCN9
• Molecular Weight: 15,427 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: TNF superfamily member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.