Mouse Tumor necrosis factor ligand superfamily member 13 ELISA Kit | TNFSF13 elisa kit

Mouse Tumor necrosis factor ligand superfamily member 13 ELISA Kit

Cat. Num. AAA765269

• Gene Names: TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715
• Reactivity: Mouse
• Synonyms: Tumor necrosis factor ligand superfamily member 13; Mouse Tumor necrosis factor ligand superfamily member 13 ELISA Kit; TNFSF13/APRIL/CD256/TALL2/TNFSF13/TRDL1/ZTNF2/APRILFLJ57090/CD256 antigen/TALL-2/TNF-related death ligand 1/TRDL-1/t tumor necrosis factor-like protein ZTNF2/tumor necrosis factor-related death ligand-1; TNFSF13 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNFSF13. No significant cross-reactivity or interference between TNFSF13 and analogues was observed.
• Sequence Length: 250

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF13 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507599
• NCBI GeneID: 8741
• NCBI Accession #: NP_003799.1
• NCBI GenBank Nucleotide #: NM_003808.3
• Molecular Weight: 36,589 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13 isoform alpha
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 13
• NCBI Official Symbol: TNFSF13
• NCBI Official Synonym Symbols: APRIL; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF13
• UniProt Synonym Gene Names: APRIL; TALL2; ZTNF2; APRIL; TALL-2; TRDL-1
• UniProt Entry Name: TNF13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; cytosol; external side of plasma membrane; extracellular region; extracellular space; nucleoplasm

Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding

Biological Process: gene expression; immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; positive regulation of isotype switching to IgA isotypes; regulation of mRNA stability; signal transduction; tumor necrosis factor-mediated signaling pathway