Mouse anti-Human GSTM1 Monoclonal Antibody | anti-GSTM1 antibody

Anti-GSTM1 Mouse mAb

Cat. Num. AAA476237

• Gene Names: GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• Reactivity: Human
• Applications: Western Blot
• Synonyms: GSTM1; Monoclonal Antibody; Anti-GSTM1 Mouse mAb; EC=2.5.1.18; Glutathione S-transferase Mu 1; GST class-mu 1; GST HB subunit 4; GST1; Gstm1; GSTM1-1; GSTM1_HUMAN; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; LIVER AND FIBROBLAST GST1; MU; MU-1; anti-GSTM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G8-C3-D5
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 218

• Applicable Applications for anti-GSTM1 antibody: Western Blot (WB)
• Immunogen: Purified recombinant fragment of human GSTM1 expressed in E Coli.
• Sequence Similarities: Contains 1 GST N-terminal domain.
• Subunit Structure: Homodimer.
• Subcellular Location: Cytoplasm

Western Blot (WB)

(Western blot detection of GSTM1 in C6, Jurkat, K562, 3T3 and Hela cell lysates using GSTM1 mouse mAb (1:2000 diluted).Predicted band size:26KDa.Observed band size:26KDa.)

Western Blot (WB)

(Western blot analysis using GSTM1 mouse mAb against MCF-7 (1), PC-12 (2), Jurkat (3), Hela (4), HL7702 (5) and HepG2 (6) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells using GSTM1 mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using GSTM1 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GSTM1 mouse mAb with DAB staining.)

Related Product Information for anti-GSTM1 antibody

Entrez Summary: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individuals susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]
UniPort Summary Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

NCBI and Uniprot Product Information

• NCBI GI #: 23065544
• NCBI GeneID: 2944
• NCBI Accession #: NP_000552.2
• NCBI GenBank Nucleotide #: NM_000561.3
• UniProt Accession #: P09488; Q5GHG0; Q6FH88; Q8TC98; Q9UC96
• Molecular Weight: 26kDa
• NCBI Official Full Name: glutathione S-transferase Mu 1 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase mu 1
• NCBI Official Symbol: GSTM1
• NCBI Official Synonym Symbols: MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
• NCBI Protein Information: glutathione S-transferase Mu 1
• UniProt Protein Name: Glutathione S-transferase Mu 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM1
• UniProt Synonym Gene Names: GST1

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol

Molecular Function: enzyme binding; glutathione binding; glutathione transferase activity; protein homodimerization activity

Biological Process: glutathione metabolic process; nitrobenzene metabolic process; xenobiotic catabolic process