TNC:TWEAK Recombinant Protein | TNC recombinant protein
TNC:TWEAK, (soluble) (mouse), (recombinant)
Gene Names
Tnfsf12; Dr3l; Apo3l; Dr3lg; Tweak
Applications
ELISA
Purity
>=95% (SDS-PAGE)
Synonyms
TNC:TWEAK; (soluble) (mouse); (recombinant); TNC recombinant protein
Host
HEK293 cells
Specificity
Binds to Fn14 (mouse):Fc (human) (rec.)
Purity/Purification
>=95% (SDS-PAGE)
Form/Format
Lyophilized. Contains PBS
Concentration
0.1mg/ml after reconstitution (varies by lot)
Applicable Applications for TNC recombinant protein
ELISA (EIA)
Source Note
The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a DYKDDDDK-tag
Endotoxin
<0.1EU/ug purified protein (LAL test)
Reconstitution
Reconstitute with 100ul sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein
Preparation and Storage
Stable for at least 6 months after receipt when stored at -20 degree C
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 degree C
Shipping: Shipped on Blue Ice
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20 degree C
Shipping: Shipped on Blue Ice
Related Product Information for TNC recombinant protein
Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor. Note: For some TNF ligands, such as FasL (mouse), activated by further oligomerization (> hexamer), the presence of TNC is not sufficient and ligand crosslinking using the "Enhancer for Ligands" is required.
Product Categories/Family for TNC recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,441 Da
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
Dr3l; Apo3l; Dr3lg; Tweak
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
Protein Family
UniProt Gene Name
Tnfsf12
UniProt Synonym Gene Names
TWEAK
UniProt Entry Name
TNF12_MOUSE
Uniprot Description
TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines (By similarity)
Protein type: Cytokine; Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis
Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; extracellular region
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: apoptosis; multicellular organismal development; positive regulation of protein catabolic process; immune response; angiogenesis; cell differentiation
Research Articles on TNC
Similar Products
Product Notes
The TNC tnfsf12 (Catalog #AAA566260) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNC:TWEAK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the TNC tnfsf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNC:TWEAK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
