Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) Standard Curve.)
Mouse Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12) ELISA Kit | TNFSF12 elisa kit
Mouse Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12) ELISA Kit
Gene Names
Tnfsf12; Dr3l; Apo3l; Dr3lg; Tweak
Reactivity
Mouse
Synonyms
Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12); Mouse Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12) ELISA Kit; TNFSF12; APO3L; DR3LG; MGC129581; MGC20669; TWEAK; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; TNFSF12 elisa kit
Reactivity
Mouse
Specificity
Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF12. No significant cross-reactivity or interference between Mouse TNFSF12 and analogues was observed.
Sequence Length
249
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) Standard Curve.)
Related Product Information for TNFSF12 elisa kit
Description: This Mouse Tumor necrosis factor ligand superfamily member 12 (TNFSF12) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF12 in samples. An antibody specific for TNFSF12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tumor necrosis factor ligand superfamily member 12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.The deduced in-frame fusion protein, which the authors called TWEPRIL, has a calculated molecular mass of 38 kD. TWEPRIL begins at the initiation codon of TWEAK and contains the intracellular, transmembrane, and stalk regions of TWEAK fused to the extracellular receptor-binding domain of APRIL.
Background: Tumor necrosis factor ligand superfamily member 12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.The deduced in-frame fusion protein, which the authors called TWEPRIL, has a calculated molecular mass of 38 kD. TWEPRIL begins at the initiation codon of TWEAK and contains the intracellular, transmembrane, and stalk regions of TWEAK fused to the extracellular receptor-binding domain of APRIL.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tumor necrosis factor ligand superfamily member 12
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol
Tnfsf12
NCBI Official Synonym Symbols
Dr3l; Apo3l; Dr3lg; Tweak
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
Protein Family
UniProt Gene Name
Tnfsf12
UniProt Synonym Gene Names
TWEAK
UniProt Entry Name
TNF12_MOUSE
Uniprot Description
TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines (By similarity)
Protein type: Cytokine; Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis
Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; extracellular region
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: apoptosis; multicellular organismal development; positive regulation of protein catabolic process; immune response; angiogenesis; cell differentiation
Research Articles on TNFSF12
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Product Notes
The Mouse TNFSF12 tnfsf12 (Catalog #AAA9716727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716727 ELISA Kit recognizes Mouse TNFSF12. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily Member 12 (TNFSF12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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