TRAIL Recombinant Protein | TNFSF10 recombinant protein

TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

Cat. Num. AAA650928

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
• Purity: Highly Purified; 95% by SDS-PAGE
• Synonyms: TRAIL; Recombinant; Human (TNF-Related Apoptosis Inducing Ligand; Apo2L; TL2; TNFSF10); TNFSF10 recombinant protein

• Host: Human, Recombinant, E Coli
• Purity/Purification: Highly Purified; 95% by SDS-PAGE
• Form/Format: Supplied as a liquid in 20mM Tris pH 7.5, 300mM NaCl, 0.1mM DTT, 10% glycerol.
• Sequence: MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNG; ELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS; KDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG

• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Related Product Information for TNFSF10 recombinant protein

TRAIL, 114-281 aa, Human, Recombinant, E. coli

Recombinant human TRAIL was expressed in E.coli and purified by using conventional chromatography techniques.
TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumor cell lines upon binding to the death-signalling receptors on the cell membrane. TRAIL binds to one of four receptors that have been identified in humans, including TRAIL-R1/DR4, TRAIL-R2/KILLER/DR5, TRAIL-R3/DcR1/TRID, and TRAIL-R4/DcR2/TRUNDD. Both DR4 and DR5 are pro-apoptotic receptors, which contain a cytoplasmic death domain and mediate apoptosis on binding to TRAIL. By contrast, TRID and TRUNDD do not contain a cytoplasmic death domain and block the function of TRAIL by competing with DR4 and DR5 for binding of TRAIL.

Product Categories/Family for TNFSF10 recombinant protein

Growth Factors; Cytokines; Growth Factors-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4507593
• NCBI GeneID: 8743
• NCBI Accession #: NP_003801.1
• NCBI GenBank Nucleotide #: NM_003810.3
• UniProt Accession #: P50591; A1Y9B3
• Molecular Weight: 23kD (211 aa)
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L
• UniProt Entry Name: TNF10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Cofactor: Binds 1 zinc ion per trimer.

Subunit structure: Homotrimer.

Subcellular location: Membrane; Single-pass type II membrane protein

Potential.

Tissue specificity: Widespread; most predominant in spleen, lung and prostate.

Sequence similarities: Belongs to the tumor necrosis factor family.