TRAIL active protein

TRAIL, 114-281aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA206638

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: TRAIL; 114-281aa; Human; E Coli (Bioactivity Validated); Tumor necrosis factor (ligand) superfamily; member 10; TNFSF10; Apo-2L; APO2L; CD253; TL2; TRAIL active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 300 mM NaCl, 0.1 mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNG ELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS KDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG
• Sequence Length: 281

• Applicable Applications for TRAIL active protein: SDS-PAGE
• Antigen Species: Human
• Biological Activity: Measured in a cell cytotoxicity assay using Jurkat human T lymphocyte. The ED50 for this effect is less or equal to 1 ng/ml
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TRAIL active protein

TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumor cell lines upon binding to the death-signalling receptors on the cell membrane. TRAIL binds to one of four receptors that have been identified in humans, including TRAIL-R1/DR4, TRAIL-R2/KILLER/DR5, TRAIL-R3/DcR1/TRID, and TRAIL-R4/DcR2/TRUNDD. Both DR4 and DR5 are pro-apoptotic receptors, which contain a cytoplasmic death domain and mediate apoptosis on binding to TRAIL. By contrast, TRID and TRUNDD do not contain a cytoplasmic death domain and block the function of TRAIL by competing with DR4 and DR5 for binding of TRAIL. Recombinant human TRAIL was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TRAIL active protein

Cancer; Apoptosis; Signal Transduction

References

Janssen EM., et al. (2005). Nature. 434 (7029):88-93.; Deocaris CC., et al. (2007) Ann N Y Acad Sci. 1119:165-75

NCBI and Uniprot Product Information

• NCBI GI #: 4507593
• NCBI GeneID: 8743
• NCBI Accession #: NP_003801
• NCBI GenBank Nucleotide #: NM_003810.3
• UniProt Accession #: P50591; A1Y9B3
• Molecular Weight: 19.6 kDa (169 aa)
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 1
• NCBI Official Synonym Full Names: TNF superfamily member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L; Protein TRAIL

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.