Typical Testing Data/Standard Curve (for reference only)
(Mouse CD30 PicoKine ELISA Kit standard curve )
Mouse CD30 ELISA Kit | TNFRSF8 elisa kit
Mouse CD30 PicoKine ELISA Kit
Gene Names
Tnfrsf8; Ki; Cd30; Ki-1; D1S166E
Reactivity
Mouse
Synonyms
CD30; Mouse CD30 PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily; member 8; CD 30; CD153; CD30 antigen; CD30L receptor; Cytokine receptor CD30; D1S166E; KI 1; Ki 1 antigen; Ki-1 antigen; KI1; Lymphocyte activation antigen CD30; TNFRSF 8; TNFRSF8; TNR8_HUMAN; Tumor Necrosis Factor Receptor Superfamily Member 8; TNFRSF8 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD30
Sequence Length
498
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD30 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD30 PicoKine ELISA Kit standard curve )
Related Product Information for TNFRSF8 elisa kit
Principle of the assay: mouse CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-T281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30 amount of sample captured in plate.
Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
53,216 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 8
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 8
NCBI Official Symbol
Tnfrsf8
NCBI Official Synonym Symbols
Ki; Cd30; Ki-1; D1S166E
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8; CD30L receptor; lymphocyte activation antigen CD30
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
Protein Family
UniProt Gene Name
Tnfrsf8
UniProt Entry Name
TNR8_MOUSE
Uniprot Description
TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Receptor, misc.; Membrane protein, integral
Cellular Component: membrane; integral to membrane
Molecular Function: transmembrane receptor activity
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; signal transduction; activation of NF-kappaB transcription factor
Research Articles on TNFRSF8
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Product Notes
The Mouse TNFRSF8 tnfrsf8 (Catalog #AAA177391) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177391 ELISA Kit recognizes Mouse TNFRSF8. It is sometimes possible for the material contained within the vial of "CD30, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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