Rabbit anti-Human DR6 Polyclonal Antibody | anti-TNFRSF21 antibody

Anti-DR6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101508

• Gene Names: TNFRSF21; DR6; CD358; BM-018
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: DR6; Polyclonal Antibody; Anti-DR6 Antibody; Rabbit Polyclonal; TNFRSF21/DR6 Antibody; Rabbit PAb; Antigen Affinity Purified; tumor necrosis factor receptor superfamily; member 21; Anti-BM-018 Antibody; Anti-CD358 Antibody; anti-TNFRSF21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TNFRSF21/DR6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TNFRSF21 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Human TNFRSF21/DR6 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human TNFRSF21/DR6 (rh TNFRSF21/DR6). TNFRSF21/DR6 specific IgG was purified by Human TNFRSF21/DR6 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-DR6 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLane B: K562 Whole Cell LysateLane C: Daudi Whole Cell LysateLane D: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:72 kDaObserved band size:60 kDa)

Related Product Information for anti-TNFRSF21 antibody

TNFRSF21 (death receptor-6, DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N-and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain, and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production, suggested that DR6 serves as an important regulatory molecule in T-helper cell activation, and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs, heart, brain and pancreas. Some tumor cells overexpress DR6, typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion, which is subject to future investigations.

Product Categories/Family for anti-TNFRSF21 antibody

Death Receptor Signaling

References

Pan G, et al. (1998) Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 431(3): 351-6.Benschop R, et al. (2009) Tumor necrosis factor receptor superfamily member 21: TNFR-related death receptor-6, DR6. Adv Exp Med Biol. 647: 186-94.Klma M, et al. (2009) Functional analysis of the posttranslational modifications of the death receptor 6. Biochim Biophys Acta. 1793(10): 1579-87.

NCBI and Uniprot Product Information

• NCBI GI #: 7657039
• NCBI GeneID: 27242
• NCBI Accession #: NP_055267.1
• NCBI GenBank Nucleotide #: NM_014452.4
• UniProt Accession #: O75509; Q0D2P5; Q96D86; B2RDI9
• Molecular Weight: 71,845 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 21
• NCBI Official Synonym Full Names: TNF receptor superfamily member 21
• NCBI Official Symbol: TNFRSF21
• NCBI Official Synonym Symbols: DR6; CD358; BM-018
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 21
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 21
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF21
• UniProt Synonym Gene Names: DR6
• UniProt Entry Name: TNR21_HUMAN

NCBI Description

This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1), and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation. [provided by RefSeq, Jul 2013]

Uniprot Description

DR6: May activate NF-kappa-B and promote apoptosis. May activate JNK and be involved in T-cell differentiation. Required for both normal cell body death and axonal pruning. Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N- terminal fragment of APP (N-APP). N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). Associates with TRADD. Interacts with N-APP. Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.

Protein type: Apoptosis; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: axon; integral to plasma membrane; plasma membrane; intrinsic to plasma membrane

Molecular Function: protein binding

Biological Process: myelination; neuron apoptosis; adaptive immune response; negative regulation of T cell proliferation; negative regulation of myelination; apoptosis; regulation of oligodendrocyte differentiation; B cell apoptosis; negative regulation of B cell proliferation; cellular lipid metabolic process; T cell receptor signaling pathway; humoral immune response