HVEM/TNFRSF14 Recombinant Protein | TNFRSF14 recombinant protein

Recombinant Human HVEM/TNFRSF14 Protein (C-mFc)

Cat. Num. AAA2557265

• Gene Names: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: HVEM/TNFRSF14; Recombinant Human HVEM/TNFRSF14 Protein (C-mFc); Tumor Necrosis Factor Receptor Superfamily Member 14; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; HveA; Tumor Necrosis Factor Receptor-Like 2; TR2; CD270;; TNFRSF14 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, 150mM NaCl, pH7.4.
• Sequence: Pro37-Val202
• Sequence Length: 283

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-mFc
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for TNFRSF14 recombinant protein

Protein Construction: Recombinant Human HVEM is produced by our mammalian expression system and the target gene encoding Pro37-Val202 is expressed with a mFc tag at the C-terminus.

Background: Herpesvirus entry mediator (HVEM) is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity. HVEM is highly expressed on na?ve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils. It functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha. Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation and contributes to Th1 inflammation and cardiac allograft rejection. In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation and dampens intestinal inflammation. HVEM enhances the development of CD8+ T cell memory and Treg function. It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding.

NCBI and Uniprot Product Information

• NCBI GI #: 13878821
• NCBI GeneID: 8764
• UniProt Accession #: Q92956
• Molecular Weight: Molecular Mass: 44.1kDa; Actual Protein Molecular Mass: 55-90kDa
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 14
• NCBI Official Synonym Full Names: TNF receptor superfamily member 14
• NCBI Official Symbol: TNFRSF14
• NCBI Official Synonym Symbols: TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 14
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 14
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF14
• UniProt Synonym Gene Names: HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
• UniProt Entry Name: TNR14_HUMAN

NCBI Description

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFRSF14: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.

Protein type: Receptor, misc.; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; entry of virus into host cell; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; T cell costimulation; positive regulation of cytokine secretion during immune response; negative regulation of alpha-beta T cell proliferation; immune response; defense response to Gram-negative bacterium