Human TNFRSF14/HVEM ELISA Kit | TNFRSF14/HVEM elisa kit

Human TNFRSF14/HVEM PicoKine Fast ELISA Kit

Cat. Num. AAA1752765

• Gene Names: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• Reactivity: Human
• Synonyms: TNFRSF14/HVEM; Human TNFRSF14/HVEM PicoKine Fast ELISA Kit; human TNFRSF14; Tumor necrosis factor receptor superfamily member 14; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; Tumor necrosis factor receptor-like 2; TR2; CD270; TNFRSF14; HVEA; HVEM; UNQ329/PRO509; Protein Tnfrsf14; TNFRSF14/HVEM elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TNFRSF14
• Sequence Length: 283

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFRSF14/HVEM elisa kit

Principle of the Assay: The Picokine™ Human TNFRSF14 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNFRSF14 with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF14. The detection antibody is a biotinylated antibody specific for TNFRSF14. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF14 with immunogen: Expression system for standard: NSO; Immunogen sequence: L39-V202. The kit is analytically validated with ready to use reagents. To measure Human TNFRSF14, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human TNFRSF14 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF14 in the sample.!!Background/Introduction: Tumor necrosis factor receptor superfamily member 14(TNFRSF14), also known as HVEM, is a protein that in humans is encoded by the TNFRSF14 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. It is mapped to 1p36.32. HVEM plays an important role in HSV pathogenesis because it enhanced the entry of several wildtype HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T cells. HVEM and BTLA which are form a bidirectional signaling pathway can regulate cell survival and inhibitory responses between interacting cells. HVEM as an important orchestrator of mucosal immunity integrates signals from innate lymphocytes to induce optimal epithelial Stat3 activation, which indicated that targeting HVEM with agonists could improve host defense.

NCBI and Uniprot Product Information

• NCBI GI #: 13878821
• NCBI GeneID: 8764
• UniProt Accession #: Q92956
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 14
• NCBI Official Synonym Full Names: TNF receptor superfamily member 14
• NCBI Official Symbol: TNFRSF14
• NCBI Official Synonym Symbols: TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 14
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 14
• UniProt Gene Name: TNFRSF14
• UniProt Synonym Gene Names: HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
• UniProt Entry Name: TNR14_HUMAN

NCBI Description

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFRSF14: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.

Protein type: Receptor, misc.; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; entry of virus into host cell; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; T cell costimulation; positive regulation of cytokine secretion during immune response; negative regulation of alpha-beta T cell proliferation; immune response; defense response to Gram-negative bacterium