Interferon Gamma Recombinant Protein | TNF recombinant protein

Recombinant Dog Interferon Gamma

Cat. Num. AAA225404

• Gene Names: TNF; TNFA; cTNF; TNLG1F
• Reactivity: Dog
• Applications: ELISA, Western Blot
• Purity: >95% by SDS PAGE analysis; Purified by ion-exchange chromatography
• Synonyms: Interferon Gamma; Recombinant Dog Interferon Gamma; IFN Gamma; TNF recombinant protein

• Host: Pichia pastoris
• Reactivity: Dog
• Purity/Purification: >95% by SDS PAGE analysis; Purified by ion-exchange chromatography
• Form/Format: Lyophilized in phosphate buffered saline with 10% Trahalose.
• Sequence Length: 233

• Applicable Applications for TNF recombinant protein: ELISA (EIA), Western Blot (WB)
• Reconstitution: Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. It is recommend that the vial is gently mixed after reconstitution.
• Preparation and Storage: Prior to reconstitution store at -20 degree C. After reconstitution store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Shelf Life: 12 months from date of despatch

ELISA (EIA)

(Dog Interferon Gamma ELISA Recombinant Dog Interferon gamma (PDP010) detected using Rabbit anti Dog interferon gamma as the capture reagent and Rabbit anti Dog Interferon gamma:Biotin as the detection reagent followed by Streptavidin:HRP)

Related Product Information for TNF recombinant protein

Recombinant Dog interferon gamma, (IFNgamma), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.Native dog interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties.

IFNgamma is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage and NK cell activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.

NCBI and Uniprot Product Information

• NCBI GI #: 1729984
• NCBI GeneID: 403922
• UniProt Accession #: P51742; Q006K5; Q28339
• Molecular Weight: 16.7 kDa
• NCBI Official Full Name: Tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: TNFA; cTNF; TNLG1F
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• Protein Family: Interferon
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

Uniprot Description

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.