Testing Data
(Bovine TNF alpha ELISARecombinant bovine TNF alpha detected using Rabbit anti Bovine TNF alpha as the capture reagent and Rabbit anti Bovine TNF alpha:Biotin as the detection reagent followed by Streptavidin:HRP)
TNF ALPHA recombinant protein
RECOMBINANT BOVINE TNF ALPHA
Gene Names
TNF; TNFa; TNF-a; TNF-alpha
Reactivity
Bovine
Applications
ELISA
Purity
>95% by SDS PAGE analysis
Synonyms
TNF ALPHA; RECOMBINANT BOVINE TNF ALPHA; TNF ALPHA recombinant protein
Reactivity
Bovine
Specificity
TNF ALPHA
Purity/Purification
>95% by SDS PAGE analysis
Form/Format
Purified recombinant protein - lyophilized
Sequence Length
234
Applicable Applications for TNF ALPHA recombinant protein
ELISA (EIA)
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Preservative Stabilisers
10% Trehalose
Buffer Solution
Phosphate buffered saline
Reconstitution
Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Preparation and Storage
Prior to reconstitution store at -20 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life: 12 months from date of despatch
Shelf Life: 12 months from date of despatch
Testing Data
(Bovine TNF alpha ELISARecombinant bovine TNF alpha detected using Rabbit anti Bovine TNF alpha as the capture reagent and Rabbit anti Bovine TNF alpha:Biotin as the detection reagent followed by Streptavidin:HRP)
Testing Data
(Bovine TNF alpha ELISARecombinant bovine TNF alpha detected using Rabbit anti Bovine TNF alpha as the capture reagent and Rabbit anti Bovine TNF alpha:Biotin as the detection reagent followed by Streptavidin:HRP)
Related Product Information for TNF ALPHA recombinant protein
Recombinant Bovine tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), corresponds to the 77 - 234 aa sequence of native bovine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified. Bovine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the bovine inflammatory response. This product may be used as a standard in immunoassays.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17.4 kDa
NCBI Official Full Name
Tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFa; TNF-a; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_BOVIN
Uniprot Description
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Research Articles on TNF ALPHA
Similar Products
Product Notes
The TNF ALPHA tnf (Catalog #AAA224992) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The RECOMBINANT BOVINE TNF ALPHA reacts with Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's TNF ALPHA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the TNF ALPHA tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF ALPHA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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