TMED1 recombinant protein

Recombinant Human TMED1 (C-Fc)

Cat. Num. AAA2569087

• Gene Names: TMED1; Tp24; Il1rl1l; IL1RL1LG
• Purity: >90% as determined by reducing SDS-PAGE
• Synonyms: TMED1; Recombinant Human TMED1 (C-Fc); IL1RL1-Binding Protein; Il1rl1l; IL1RL1LG; IL-1RL1LG; IL1RL1LGIL1RL1-binding protein; ST2L; T1/ST2 receptor binding protein; Tp24; TMED1 recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, pH7.4.
• Sequence: Ala24-Asn194

• Species: Human
• Tag: C-Fc
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Transmembrane Emp24 Domain-containing Protein 1 is produced by our Mammalian expression system and the target gene encoding Ala24-Asn194 is expressed with a Fc tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for TMED1 recombinant protein

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking. TMED1 is a 59kDa monomer and has been reported to exist as homodimer. It contains 1 GOLD domain and is widely expressed. TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL 33. This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK. Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions.

Product Categories/Family for TMED1 recombinant protein

Cell biology

NCBI and Uniprot Product Information

• NCBI GI #: 5803040
• NCBI GeneID: 11018
• NCBI Accession #: NP_006849.1
• NCBI GenBank Nucleotide #: NM_006858.3
• UniProt Accession #: Q13445
• Molecular Weight: 25,206 Da
• NCBI Official Full Name: transmembrane emp24 domain-containing protein 1
• NCBI Official Synonym Full Names: transmembrane emp24 protein transport domain containing 1
• NCBI Official Symbol: TMED1
• NCBI Official Synonym Symbols: Tp24; Il1rl1l; IL1RL1LG
• NCBI Protein Information: transmembrane emp24 domain-containing protein 1; IL1RL1-binding protein; p24 family protein gamma-1; interleukin 1 receptor-like 1 ligand; putative T1/ST2 receptor-binding protein
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 1
• Protein Family: Transmembrane emp24 domain-containing protein
• UniProt Gene Name: TMED1
• UniProt Synonym Gene Names: IL1RL1L; IL1RL1LG; Tp24; p24gamma1
• UniProt Entry Name: TMED1_HUMAN

NCBI Description

This gene belongs to the TMED (transmembrane emp24 domain-containing) protein family, which is involved in the vesicular trafficking of proteins. The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1) and may play a role in innate immunity. This protein lacks any similarity to other interleukin 1 ligands. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

TMED1: Potential role in vesicular protein trafficking, mainly in the early secretory pathway. May act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; plasma membrane; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; receptor binding

Biological Process: protein transport; cell-cell signaling; signal transduction