TMED1 active protein

TMED1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121372

• Gene Names: TMED1; Tp24; Il1rl1l; IL1RL1LG
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TMED1; TMED1 Protein; Human; Recombinant (His Tag); Human TMED1 Protein (His Tag); Il1rl1l Protein; IL1RL1LG Protein; Tp24 Protein; transmembrane emp24 protein transport domain containing 1; TMED1 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn194

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TMED1 (Q13445)(Met1-Asn194) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1.Measured by its binding ability in a functional ELISA.2.Immobilized human TMED1-His at 10ug/mL (100uL/well) can bind human IL1R4-Fc. The EC50 of human IL1R4-Fc is 8-50ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human TMED1-His at 10 ug/mL (100 uL/well) can bind human IL1R4-Fc, the EC50 of human IL1R4-Fc is 8-50 ng/mL.)

Related Product Information for TMED1 active protein

Background: TMED1 belongs to the EMP24/GP25L family. It contains 1 GOLD domain and is widely expressed. TMED1 binds to its receptor IL1RL1 and results in the activation of DNA binding by nuclear factor NF-kappa-B or transcription from the IL8 promoter and most likely requires other proteins to elicit these activities. Dendritic cells from Peyer's patches (but not from spleen) express TMED1 in response to treatment with LPS. TMED1 may play a role in vesicular protein trafficking, mainly in the early secretory pathway. It may act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.

References

Colland F, et al. (2004) Functional Proteomics Mapping of a Human Signaling Pathway. Genome Res. 14(7):1324-32.Gerhard DS, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8.Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.

NCBI and Uniprot Product Information

• NCBI GI #: 5803040
• NCBI GeneID: 11018
• NCBI Accession #: NP_006849.1
• NCBI GenBank Nucleotide #: NM_006858.3
• UniProt Accession #: Q13445
• Molecular Weight: 25,206 Da
• NCBI Official Full Name: transmembrane emp24 domain-containing protein 1
• NCBI Official Synonym Full Names: transmembrane emp24 protein transport domain containing 1
• NCBI Official Symbol: TMED1
• NCBI Official Synonym Symbols: Tp24; Il1rl1l; IL1RL1LG
• NCBI Protein Information: transmembrane emp24 domain-containing protein 1; IL1RL1-binding protein; p24 family protein gamma-1; interleukin 1 receptor-like 1 ligand; putative T1/ST2 receptor-binding protein
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 1
• Protein Family: Transmembrane emp24 domain-containing protein
• UniProt Gene Name: TMED1
• UniProt Synonym Gene Names: IL1RL1L; IL1RL1LG; Tp24; p24gamma1
• UniProt Entry Name: TMED1_HUMAN

NCBI Description

This gene belongs to the TMED (transmembrane emp24 domain-containing) protein family, which is involved in the vesicular trafficking of proteins. The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1) and may play a role in innate immunity. This protein lacks any similarity to other interleukin 1 ligands. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

TMED1: Potential role in vesicular protein trafficking, mainly in the early secretory pathway. May act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; plasma membrane; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; receptor binding

Biological Process: protein transport; cell-cell signaling; signal transduction