3,3',5,5'-Tetramethylbenzidine Reagent | TMB reagent
3,3',5,5'-Tetramethylbenzidine (TMB) Substrate (1-Component) for ELISA
Synonyms
3; 3'; 5; 5'-Tetramethylbenzidine; 5'-Tetramethylbenzidine (TMB) Substrate (1-Component) for ELISA; TMB reagent
Protocol
1. Add 100ul of this product into each well.
2. Cover the plate and incubate at 37°C in dark within 30min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated HRP), the other wells show no obvious color.
3. Add 100ul of the stop solution 0.1% NaF or 0.3M H2SO4 or 0.25M HCl into each well.
4. Read the O.D Absorbance at 650nm if use 0.1% NaF as the stop solution, or at 450nm if use 0.3M H2SO4 or 0.25M HCl as the stop solution.
2. Cover the plate and incubate at 37°C in dark within 30min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated HRP), the other wells show no obvious color.
3. Add 100ul of the stop solution 0.1% NaF or 0.3M H2SO4 or 0.25M HCl into each well.
4. Read the O.D Absorbance at 650nm if use 0.1% NaF as the stop solution, or at 450nm if use 0.3M H2SO4 or 0.25M HCl as the stop solution.
Note
1. This product is sensitive to oxidizng agent, end users should ensure to avoid any possible contamination.
2. Use this product in dark, it is better to use the plastic containers to subpackage, do NOT use the metal or contaminated containers.
3. High concentration of HRP may lead to the TMB turns to green or red, or produce sediment, in this way, end users should decrease the amount of antibodies or HRP conjugations or reduce the incubation time.
2. Use this product in dark, it is better to use the plastic containers to subpackage, do NOT use the metal or contaminated containers.
3. High concentration of HRP may lead to the TMB turns to green or red, or produce sediment, in this way, end users should decrease the amount of antibodies or HRP conjugations or reduce the incubation time.
Preparation and Storage
Store at 2-8°C for 2 years.
Related Product Information for TMB reagent
1. The main component for this poroduct is 3,3',5,5'-Tetramethylbenzidine, TMB, it was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product. The density of blue is proportional to HRP activity.
2. It is ready to use substrate with one component, very stable for convenient use.
2. It is ready to use substrate with one component, very stable for convenient use.
Product Categories/Family for TMB reagent
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Product Notes
The TMB (Catalog #AAA355736) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "3,3',5,5'-Tetramethylbenzidine, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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