Human Toll Like Receptor 9 ELISA Kit | TLR9 elisa kit

Human Toll Like Receptor 9 ELISA Kit

Cat. Num. AAA751526

• Reactivity: Human
• Synonyms: Toll Like Receptor 9; Human Toll Like Receptor 9 ELISA Kit; TLR9 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This TLR9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TLR9. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR9 elisa kit

Principle of the Assay: TLR9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR9 antibody and an TLR9-HRP conjugate. The assay sample and buffer are incubated together with TLR9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR9 concentration since TLR9 from samples and TLR9-HRP conjugate compete for the anti-TLR9 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR9 from the sample, fewer sites are left to bind TLR9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TLR9 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 29420453
• NCBI GeneID: 397007
• UniProt Accession #: Q5I2M3
• Molecular Weight: 115,883 Da
• NCBI Official Full Name: Toll like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR9
• UniProt Entry Name: TLR9_PIG

Uniprot Description

Function: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response

By similarity.

Subunit structure: Interacts with MYD88 via their respective TIR domains

By similarity. Interacts with BTK

By similarity. Interacts (via transmembrane domain) with UNC93B1

By similarity. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses

By similarity. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein

By similarity. Endosome

By similarity. Lysosome

By similarity. Cytoplasmic vesicle › phagosome

By similarity. Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist

By similarity.

Sequence similarities: Belongs to the Toll-like receptor family.Contains 26 LRR (leucine-rich) repeats.Contains 1 TIR domain.