Rabbit anti-Mouse Rad50 Polyclonal Antibody | anti-Rad50 antibody

Rad50, CT (Rad50, DNA repair protein RAD50) (Azide free) (HRP)

Cat. Num. AAA6340013

• Gene Names: Rad50; Mrell; Rad50l
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Rad50; Polyclonal Antibody; CT (Rad50; DNA repair protein RAD50) (Azide free) (HRP); DNA repair protein RAD50; anti-Rad50 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1312

• Applicable Applications for anti-Rad50 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Rad50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 910-938 amino acids from the C-terminal region of mouse Rad50.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Rad50 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Rad50 antibody detected the Rad50 protein (arrow).)

Related Product Information for anti-Rad50 antibody

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity).

Product Categories/Family for anti-Rad50 antibody

Antibodies; DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 60392985
• NCBI GeneID: 19360
• UniProt Accession #: P70388
• NCBI Official Full Name: DNA repair protein RAD50
• NCBI Official Synonym Full Names: RAD50 double strand break repair protein
• NCBI Official Symbol: Rad50
• NCBI Official Synonym Symbols: Mrell; Rad50l
• NCBI Protein Information: DNA repair protein RAD50
• UniProt Protein Name: DNA repair protein RAD50
• Protein Family: RAD50-interacting protein
• UniProt Gene Name: Rad50
• UniProt Synonym Gene Names: mRad50

Uniprot Description

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation ().