Human Toll-like receptor 7 ELISA Kit | TLR7 elisa kit

Human Toll-like receptor 7 ELISA Kit

Cat. Num. AAA2883924

• Gene Names: TLR7; TLR7-like
• Reactivity: Human
• Synonyms: Toll-like receptor 7; Human Toll-like receptor 7 ELISA Kit; TLR7; UNQ248/PRO285; TLR7 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Toll-like receptor 7
• Sequence Length: 1049

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.09 ng/mL
• Intra-assay Precision: <=5.2%
• Inter-assay Precision: <=9.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR7 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7706093
• NCBI GeneID: 51284
• NCBI Accession #: NP_057646.1
• NCBI GenBank Nucleotide #: NM_016562.3
• UniProt Accession #: Q9NYK1; Q9NR98; D1CS69
• Molecular Weight: 120,922 Da
• NCBI Official Full Name: toll-like receptor 7
• NCBI Official Synonym Full Names: toll like receptor 7
• NCBI Official Symbol: TLR7
• NCBI Official Synonym Symbols: TLR7-like
• NCBI Protein Information: toll-like receptor 7
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR7
• UniProt Entry Name: TLR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; endosome; endosome membrane; Golgi membrane; integral to membrane; lysosome; plasma membrane; receptor complex

Molecular Function: double-stranded RNA binding; drug binding; single-stranded RNA binding; siRNA binding; transmembrane receptor activity

Biological Process: defense response to virus; I-kappaB phosphorylation; inflammatory response; innate immune response; microglial cell activation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of chemokine production; positive regulation of inflammatory response; positive regulation of interferon-alpha biosynthetic process; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of NF-kappaB import into nucleus; regulation of protein amino acid phosphorylation; toll-like receptor 7 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway