TLK2 / PKU-ALPHA recombinant protein

Recombinant Human TLK2 / PKU-ALPHA Protein (aa 397-772, His & GST tag)

Cat. Num. AAA2545063

• Gene Names: TLK2; HsHPK; PKU-ALPHA
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TLK2 / PKU-ALPHA; Recombinant Human TLK2 / PKU-ALPHA Protein (aa 397-772; His & GST tag); MGC44450; PKU-ALPHA; TLK2 / PKU-ALPHA recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, pH 8.0
• Sequence Length: 772

• Application Notes: The recombinant human TLK2 (aa 397-772)/GST chimera consists of 613 amino acids and has a calculated molecular mass of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TLK2 / PKU-ALPHA recombinant protein

Background: Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to the protein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains one protein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.

Description: A DNA sequence encoding the amino acids (Leu 397-Asn 772) of human TLK2 (Q86UE8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 547235496
• NCBI GeneID: 11011
• NCBI Accession #: NP_001271262.1
• NCBI GenBank Nucleotide #: NM_001284333.1
• UniProt Accession #: Q86UE8; Q9UKI7; Q9Y4F7; D3DU07
• Molecular Weight: 82,348 Da
• NCBI Official Full Name: serine/threonine-protein kinase tousled-like 2 isoform C
• NCBI Official Synonym Full Names: tousled like kinase 2
• NCBI Official Symbol: TLK2
• NCBI Official Synonym Symbols: HsHPK; PKU-ALPHA
• NCBI Protein Information: serine/threonine-protein kinase tousled-like 2
• UniProt Protein Name: Serine/threonine-protein kinase tousled-like 2
• UniProt Gene Name: TLK2

NCBI Description

This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

TLK2: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); TLK family

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: cell junction; intermediate filament; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: chromosome segregation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of chromatin assembly or disassembly; response to DNA damage stimulus