TK1 recombinant protein

TK1, 1-234aa, Human, His tag, E Coli

Cat. Num. AAA204448

• Gene Names: TK1; TK2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TK1; 1-234aa; Human; His tag; E Coli; Thymidine kinase cytosolic; Thymidine kinase; cytosolic; TK2; TK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-234aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN

• Applicable Applications for TK1 recombinant protein: SDS-PAGE
• Tag Information: His-tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for TK1 recombinant protein

TK1, also known as thymidine kinase, is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. It is present in two forms in mammalian cells, TK1 and TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Deoxythymidine is present in the body fluids as a result of degradation of DNA from food and from dead cells. Thymidine kinase is required for the action of many antiviral drugs. It is used to select hybridoma cell lines in production of monoclonal antibodies. In clinical chemistry it is used as a proliferation marker in the identification, control of treatment and follow-up of malignant disease, mainly of hematological malignancies. Recombinant human TK1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for TK1 recombinant protein

Epigenomics

References

Chang Z.F., et al. (1998) J. Biol. Chem. 273:12095-12100; Kreidberg J.A., et al. (1986) Mol. Cell. Biol. 6:2903-2909

NCBI and Uniprot Product Information

• NCBI GI #: 164698438
• NCBI GeneID: 7083
• NCBI Accession #: NP_003249.3
• NCBI GenBank Nucleotide #: NM_003258.4
• UniProt Accession #: P04183; Q969V0; Q9UMG9; B2RC58
• Molecular Weight: 28.0 kDa (258aa) confirmed by MALDI-TOF
• NCBI Official Full Name: thymidine kinase, cytosolic isoform 1
• NCBI Official Synonym Full Names: thymidine kinase 1
• NCBI Official Symbol: TK1
• NCBI Official Synonym Symbols: TK2
• NCBI Protein Information: thymidine kinase, cytosolic
• UniProt Protein Name: Thymidine kinase, cytosolic
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK1

NCBI Description

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

Uniprot Description

MiscellaneousTwo forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.