Tissue Inhibitor of Metalloproteinases 1, Western Blot Control Recombinant Protein
Tissue Inhibitor of Metalloproteinases 1, Recombinant, Mouse, Western Blot Control (TIMP1, Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)
Applications
ELISA, Western Blot, Gel Super Shift Assay
Purity
PurifiedPurified
Synonyms
Tissue Inhibitor of Metalloproteinases 1; Western Blot Control; Recombinant; Mouse; Western Blot Control (TIMP1; Erythroid Potentiating Activity; EPA; Collagenase Inhibitor; CI); Western Blot Control recombinant protein
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a lyophilized powder from conditioned media.
Applicable Applications for Tissue Inhibitor of Metalloproteinases 1, Western Blot Control recombinant protein
ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Enzymatic Assays, ELISA, Western Blot and Substrate Gel Analysis.
Dilution: Western Blot: Resuspend the lyophilized powder in 250ul SDS-PAGE sample buffer. Load 10ul per lane. Standard can be detected as a 32kD protein under reducing conditions and as a 30kD protein under non-reducing conditions.
Dilution: Western Blot: Resuspend the lyophilized powder in 250ul SDS-PAGE sample buffer. Load 10ul per lane. Standard can be detected as a 32kD protein under reducing conditions and as a 30kD protein under non-reducing conditions.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Tissue Inhibitor of Metalloproteinases 1, Western Blot Control recombinant protein
TIMP-1 is the first described and best characterized of the four known mammalian TIMPs. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMP-2 has a greater efficiency against MMP-2, and TIMP-1 works better against MMP-9, MMP-1 and MMP-3. The function of the ADAMs (A Disintigrin And Metalloproteinase) proteinases is getting more attention, and it has been shown that TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-1 seems to be much less active on ADAM-17 than is TIMP-3. The TIMP-1 is largely the glycosylated form, about 29kD in size on reduced Western Blots.
T5580-06B is recombinant mouse tissue inhibitor of metalloproteinases-1 (rmTIMP-1) with a histidine tag.
T5580-06B is recombinant mouse tissue inhibitor of metalloproteinases-1 (rmTIMP-1) with a histidine tag.
Product Categories/Family for Tissue Inhibitor of Metalloproteinases 1, Western Blot Control recombinant protein
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Product Notes
The Tissue Inhibitor of Metalloproteinases 1, Western Blot Control (Catalog #AAA638014) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tissue Inhibitor of Metalloproteinases 1, Western Blot Control can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in Enzymatic Assays, ELISA, Western Blot and Substrate Gel Analysis. Dilution: Western Blot: Resuspend the lyophilized powder in 250ul SDS-PAGE sample buffer. Load 10ul per lane. Standard can be detected as a 32kD protein under reducing conditions and as a 30kD protein under non-reducing conditions. Researchers should empirically determine the suitability of the Tissue Inhibitor of Metalloproteinases 1, Western Blot Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tissue Inhibitor of Metalloproteinases 1, Western Blot Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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