CD30/TNFRSF8 recombinant protein

Recombinant mouse CD30/TNFRSF8 protein

Cat. Num. AAA201263

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: CD30/TNFRSF8; Recombinant mouse CD30/TNFRSF8 protein; Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Lymphocyte activation antigen CD30; CD_antigen; CD30; Ki-1; D1S166E; ki; CD30/TNFRSF8 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-258aa
• Sequence: FPTDRPLKTT CAGDLSHYPG EAARNCCYQC PSGLSPTQPC PRGPAHCRKQ CAPDYYVNED GKCTACVTCL PGLVEKAPCS GNSPRICECQ PGMHCCTPAV NSCARCKLHC SGEEVVKSPG TAKKDTICEL PSSGSGPNCS NPGDRKTLTS HATPQAMPTL ESPANDSARS LLPMRVTNLV QEDATELVKV PESSSSKARE PSPDPGNAEK NMTLELPSPG TLPDISTSEN SKEPASTAST

• Applicable Applications for CD30/TNFRSF8 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: hIgG-His-Tag
• Predicted N-term: Phe 19
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CD30/TNFRSF8 recombinant protein

CD30/TNFRSF8, also known as TNF Receptor Superfamily Member 8, is a member of TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells. In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5. Recombinant mouse CD30/TNFRSF8 protein, fused to hIgG-His tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD30/TNFRSF8 recombinant protein

Immunology; Proteins

References

So T, Ishii N. (2019) Adv Exp Med Biol. 1189:53-84. Jeon SH, et al, (2015) Acta Odontol Scand. 73(8):588-594.

NCBI and Uniprot Product Information

• UniProt Accession #: Q60846
• Molecular Weight: 52.2kDa (479aa)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 8
• UniProt Gene Name: Tnfrsf8
• UniProt Entry Name: TNR8_MOUSE

Uniprot Description

TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: membrane; integral to membrane

Molecular Function: transmembrane receptor activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; signal transduction; activation of NF-kappaB transcription factor