Thyroid hormone receptor alpha (THRA) Recombinant Protein | THRA recombinant protein

Recombinant Human Thyroid hormone receptor alpha (THRA)

Cat. Num. AAA7135791

• Gene Names: THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Thyroid hormone receptor alpha (THRA); Recombinant Human Thyroid hormone receptor alpha (THRA); Nuclear receptor subfamily 1 group A member 1 (V-erbA-related protein 7); (EAR-7); (c-erbA-1); (c-erbA-alpha); (EAR7); (ERBA1); (NR1A1); (THRA1); (THRA2); THRA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid or Lyophilized powder; If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
• Sequence Positions: 1-490aa; Full Length
• Sequence: MEQKPSKVECGSDPEENSARSPDGKRKRKNGQCSLKTSMSGYIPSYLDKDEQCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPTYSCKYDSCCVIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEQNRERRRKEEMIRSLQQRPEPTPEEWDLIHIATEAHRSTNAQGSHWKQRRKFLPDDIGQSPIVSMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPESDTLTLSGEMAVKREQLKNGGLGVVSDAIFELGKSLSAFNLDDTEVALLQAVLLMSTDRSGLLCVDKIEKSQEAYLLAFEHYVNHRKHNIPHFWPKLLMKEREVQSSILYKGAAAEGRPGGSLGVHPEGQQLLGMHVVQGPQVRQLEQQLGEAGSLQGPVLQHQSPKSPQQRLLELLHRSGILHARAVCGEDDSSEADSPSSSEEEPEVCEDLAGNAASP
• Sequence Length: 451

• Species: Human
• Tag: N-terminal 6xHis-tagged
• Expression System: E.coli
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. ; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.; The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.; Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Product Categories/Family for THRA recombinant protein

Neuroscience

References

"Characterization of a third human thyroid hormone receptor coexpressed with other thyroid hormone receptors in several tissues."
Nakai A., Sakurai A., Bell G.I., Degroot L.J.Mol. Endocrinol. 2:1087-1092(1988)

NCBI and Uniprot Product Information

• NCBI GI #: 300116307
• NCBI GeneID: 7067
• NCBI Accession #: NP_001177847.1
• NCBI GenBank Nucleotide #: NP_001177847.1
• UniProt Accession #: P10827
• Molecular Weight: 58.9kDa
• NCBI Official Full Name: thyroid hormone receptor alpha isoform 3
• NCBI Official Synonym Full Names: thyroid hormone receptor alpha
• NCBI Official Symbol: THRA
• NCBI Official Synonym Symbols: AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• NCBI Protein Information: thyroid hormone receptor alpha
• UniProt Protein Name: Thyroid hormone receptor alpha
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: THRA
• UniProt Synonym Gene Names: EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
• UniProt Entry Name: THA_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein binding; zinc ion binding; chromatin DNA binding; sequence-specific DNA binding; TATA-binding protein binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; cytoplasmic sequestering of transcription factor; ossification; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation

Disease: Hypothyroidism, Congenital, Nongoitrous, 6