Rabbit anti-Human TNFSF14 Polyclonal Antibody | anti-TNFSF14 antibody

TNFSF14, ID (TNFSF14, HVEML, LIGHT, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, CD258, Tumor necrosis factor ligand superfamily member 14, membrane form, Tumor necrosis factor ligand superfamily member 14, solub

Cat. Num. AAA6357036

• Gene Names: TNFSF14; LTg; TR2; CD258; HVEML; LIGHT
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TNFSF14; Polyclonal Antibody; ID (TNFSF14; HVEML; LIGHT; Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; CD258; membrane form; solub; soluble form; anti-TNFSF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TNFSF14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TNFSF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the Central region of human TNFSF14.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TNFSF14 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TNFSF14 antibody detected the TNFSF14 protein (arrow).)

Related Product Information for anti-TNFSF14 antibody

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a Herpes virus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by Herpes virus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Product Categories/Family for anti-TNFSF14 antibody

Antibodies; Growth Factors; Cytokines

References

Stiles, K.M., et al. J. Virol. 84(22):11646-11660(2010). Jin, H.R., et al. Biochem. Biophys. Res. Commun. 400(4):581-586(2010). Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Cheung, T.C., et al. J. Immunol. 185(3):1949-1958(2010). Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 291045244
• NCBI GeneID: 8740
• NCBI Accession #: NP_742011.2; NP_003798.2
• NCBI GenBank Nucleotide #: NM_172014.2
• UniProt Accession #: O43557; O75476; Q6FHA1; Q8WVF8; Q96LD2; A8K7M2; C9J5H4
• Molecular Weight: 20.1 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 14 isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 14
• NCBI Official Symbol: TNFSF14
• NCBI Official Synonym Symbols: LTg; TR2; CD258; HVEML; LIGHT
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 14
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 14
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF14
• UniProt Synonym Gene Names: HVEML; LIGHT; HVEM-L
• UniProt Entry Name: TNF14_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine; Inhibitor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein binding; caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: release of cytoplasmic sequestered NF-kappaB; T cell proliferation; T cell activation; apoptosis; T cell costimulation; T cell homeostasis; immune response; negative regulation of caspase activity; signal transduction; positive regulation of myoblast differentiation