Thrombopoietin Recombinant Protein | THPO recombinant protein

Recombinant Mouse Thrombopoietin Protein

Cat. Num. AAA8305342

• Gene Names: Thpo; Ml; Tpo; Mgdf; Mpllg
• Applications: ELISA, Western Blot, SDS-Page
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Thrombopoietin; Recombinant Mouse Thrombopoietin Protein; MGDF; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Myeloproliferative leukemia virus oncogene ligand; THPO recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: HHHHHHSPVA PACDPRLLNK LLRDSHLLHS RLSQCPDVDP LSIPVLLPAV DFSLGEWKTQ TEQSKAQDIL GAVSLLLEGV MAARGQLEPS CLSSLLGQLS GQVRLLLGAL QGLLGTQLPL QGRTTAHKDP NALFLSLQQL LRGKVRFLLL VEGPTLCVRR TLPTTAVPSS TSQLLTLNKF PNRTSGLLET NFSVTARTAG PGLLSRLQGF RVKITPGQLN QTSRSPVQIS GYLNRTHGPV NGTHGLFAGT SLQTLEASDI SPGAFNKGSL AFNLQGGLPP SPSLAPDGHT PFPPSPALPT THGSPPQLHP LFPDPSTTMP NSTAPHPVTM YPHPRNLSQE T
• Sequence Length: 356

• Applicable Applications for THPO recombinant protein: ELISA (EIA), Western Blot (WB), SDS-PAGE
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for THPO recombinant protein

Recombinant Mouse Thrombopoietin Protein is produced by mammalian expression system and the target gene encoding Ser22-Thr356 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 291045237
• NCBI GeneID: 21832
• NCBI Accession #: NP_001166976.1
• NCBI GenBank Nucleotide #: NP_001166976.1
• UniProt Accession #: P40226
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: Thpo
• NCBI Official Synonym Symbols: Ml; Tpo; Mgdf; Mpllg
• NCBI Protein Information: thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: Thpo
• UniProt Synonym Gene Names: ML; MGDF
• UniProt Entry Name: TPO_MOUSE

NCBI Description

This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; cytokine activity; receptor binding

Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation