Mouse TPO ELISA Kit | TPO elisa kit

Mouse TPO PicoKine ELISA Kit

Cat. Num. AAA175907

• Gene Names: Thpo; Ml; Tpo; Mgdf; Mpllg
• Reactivity: Mouse
• Synonyms: TPO; Mouse TPO PicoKine ELISA Kit; Thrombopoietin; thyroid peroxidase; C mpl ligand; Megakaryocyte colony stimulating factor; Megakaryocyte growth and development factor; Megakaryocyte stimulating factor; MGC163194; MGDF; MKCSF; ML; MPL ligand; MPLLG; Myeloproliferative leukemia virus oncogene ligand; OTTHUMP00000210504; THPO; TPO elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse TPO
• Sequence Length: 356

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse TPO PicoKine ELISA Kit standard curve )

Related Product Information for TPO elisa kit

Principle of the assay: mouse TPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPO has been precoated onto 96-well plates. Standards(NSO,S22-T356) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TPO amount of sample captured in plate.
Background: Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mousecDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acidswith the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 730983
• NCBI GeneID: 21832
• UniProt Accession #: P40226; JC4227
• Molecular Weight: 37,384 Da
• NCBI Official Full Name: Thrombopoietin
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: Thpo
• NCBI Official Synonym Symbols: Ml; Tpo; Mgdf; Mpllg
• NCBI Protein Information: thrombopoietin; C-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: Thpo
• UniProt Synonym Gene Names: ML; MGDF
• UniProt Entry Name: TPO_MOUSE

NCBI Description

This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Cell cycle regulation

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; cytokine activity; receptor binding

Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation