Thrombospondin 1 (THBS1) Recombinant Protein | THBS1 recombinant protein

Recombinant Thrombospondin 1 (THBS1)

Cat. Num. AAA2010383

• Gene Names: Thbs1; TSP1; TSP-1; tbsp1; Thbs-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thrombospondin 1 (THBS1); Recombinant Thrombospondin 1 (THBS1); THBS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- RSRLCN NPTPQFGGKD CVGDVTENQV CNKQDCPIDG CLSNPCFAGA KCTSYPDGSW KCGACPPGYS GNGIQCKDVD ECKEVPDACF NHNGEHRCKN TDPGYNCLPC PPRFTGSQPF GRGVEHAMAN KQVCKPRNPC TDGTHDCNKN AKCNYLGHYS D
• Sequence Length: 1170

• Applicable Applications for THBS1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg515~Asp671 (Accession # P35441) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 549134
• NCBI GeneID: 21825
• UniProt Accession #: P35441
• Molecular Weight: 18.5kDa
• NCBI Official Full Name: Thrombospondin-1
• NCBI Official Synonym Full Names: thrombospondin 1
• NCBI Official Symbol: Thbs1
• NCBI Official Synonym Symbols: TSP1; TSP-1; tbsp1; Thbs-1
• NCBI Protein Information: thrombospondin-1
• UniProt Protein Name: Thrombospondin-1
• Protein Family: Thrombospondin
• UniProt Gene Name: Thbs1
• UniProt Synonym Gene Names: Tsp1
• UniProt Entry Name: TSP1_MOUSE

NCBI Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis; Inhibitor

Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum; cytoplasm; fibrinogen complex; extracellular region; secretory granule; external side of plasma membrane

Molecular Function: integrin binding; heparin binding; protein binding; fibroblast growth factor binding; extracellular matrix binding; phosphatidylserine binding; transforming growth factor beta binding; fibronectin binding; low-density lipoprotein binding; laminin binding; calcium ion binding

Biological Process: positive regulation of blood coagulation; positive regulation of translation; activation of MAPK activity; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell adhesion; inflammatory response; cell cycle arrest; positive regulation of macrophage activation; positive regulation of tumor necrosis factor biosynthetic process; negative regulation of interleukin-12 production; cell migration; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; negative regulation of cell-matrix adhesion; negative regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; negative regulation of angiogenesis; negative regulation of dendritic cell antigen processing and presentation; response to mechanical stimulus; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; regulation of cGMP metabolic process; peptide cross-linking; sprouting angiogenesis; response to calcium ion; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cell migration