Human Chymotrypsin-Like Elastase Family Member 3B (ELA3B) ELISA Kit | ELA3B elisa kit

Human Chymotrypsin-Like Elastase Family Member 3B (ELA3B) ELISA Kit

Cat. Num. AAA9716064

• Gene Names: CELA3B; CBPP; ELA3B
• Reactivity: Human
• Synonyms: Chymotrypsin-Like Elastase Family Member 3B (ELA3B); Human Chymotrypsin-Like Elastase Family Member 3B (ELA3B) ELISA Kit; ELA3B; RP11-26H16.3; CBPP; E1; EL-1; cholesterol-binding pancreatic protease; elastase 1; elastase 3B; pancreatic; fecal elastase 1; pancreatic elastase 1; pancreatic endopeptidase E; protease E; proteinas; ELA3B elisa kit

• Reactivity: Human
• Specificity: Human Chymotrypsin-like elastase family member 3B (ELA3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ELA3B. No significant cross-reactivity or interference between Human ELA3B and analogues was observed.
• Sequence Length: 270

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Chymotrypsin-like elastase family member 3B (ELA3B) Standard Curve.)

Related Product Information for ELA3B elisa kit

Description: This Human Chymotrypsin-like elastase family member 3B (ELA3B) ELISA Kit employs a two-site sandwich ELISA to quantitate ELA3B in samples. An antibody specific for ELA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays..

NCBI and Uniprot Product Information

• NCBI GI #: 6679625
• NCBI GeneID: 23436
• NCBI Accession #: NP_031378
• NCBI GenBank Nucleotide #: NP_031378.1
• UniProt Accession #: P08861
• NCBI Official Full Name: chymotrypsin-like elastase family member 3B preproprotein
• NCBI Official Synonym Full Names: chymotrypsin like elastase 3B
• NCBI Official Symbol: CELA3B
• NCBI Official Synonym Symbols: CBPP; ELA3B
• NCBI Protein Information: chymotrypsin-like elastase family member 3B
• UniProt Protein Name: Chymotrypsin-like elastase family member 3B
• UniProt Gene Name: CELA3B
• UniProt Synonym Gene Names: ELA3B
• UniProt Entry Name: CEL3B_HUMAN

NCBI Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq, May 2009]