Thrombospondin 1 (THBS1) Recombinant Protein | THBS1 recombinant protein

Recombinant Thrombospondin 1 (THBS1)

Cat. Num. AAA2011318

• Gene Names: THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thrombospondin 1 (THBS1); Recombinant Thrombospondin 1 (THBS1); THBS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-SGGDNSV FDIFELTGAA RKGSGRRLVK GPDPSSPAFR IEDANLIPPV PDDKFQDLVD AVRAEKGFLL LASLRQMKKT RGTLLALERK DHSGQVFSVVSNGKAGTLDL SLTVQGKQHV VSVEEALLAT GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSVFTRD LASIARLRIA KGGVNDNFQG VLQNVRFVFG T
• Sequence Length: 1170

• Applicable Applications for THBS1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser24~Thr221 (Accession # P07996) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40317626
• NCBI GeneID: 7057
• NCBI Accession #: NP_003237.2
• NCBI GenBank Nucleotide #: NM_003246.2
• UniProt Accession #: P07996; Q15667; A8K6H4; B9EGH6
• Molecular Weight: 23.1kDa
• NCBI Official Full Name: thrombospondin-1
• NCBI Official Synonym Full Names: thrombospondin 1
• NCBI Official Symbol: THBS1
• NCBI Official Synonym Symbols: TSP; THBS; TSP1; TSP-1; THBS-1
• NCBI Protein Information: thrombospondin-1; thrombospondin-1p180
• UniProt Protein Name: Thrombospondin-1
• Protein Family: Thrombospondin
• UniProt Gene Name: THBS1
• UniProt Synonym Gene Names: TSP; TSP1
• UniProt Entry Name: TSP1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family.

Protein type: Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: extracellular matrix; extracellular space; cell surface; sarcoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; fibrinogen complex; extracellular region; external side of plasma membrane; secretory granule

Molecular Function: integrin binding; heparin binding; identical protein binding; proteoglycan binding; protein binding; fibroblast growth factor binding; transforming growth factor beta binding; phosphatidylserine binding; fibronectin binding; low-density lipoprotein binding; laminin binding; calcium ion binding; glycoprotein binding

Biological Process: extracellular matrix organization and biogenesis; activation of MAPK activity; response to magnesium ion; negative regulation of fibrinolysis; response to glucose stimulus; cell cycle arrest; cell adhesion; positive regulation of macrophage activation; response to drug; platelet activation; negative regulation of interleukin-12 production; positive regulation of chemotaxis; positive regulation of blood vessel endothelial cell migration; response to testosterone stimulus; negative regulation of blood vessel endothelial cell migration; negative regulation of cell-matrix adhesion; response to unfolded protein; positive regulation of angiogenesis; response to mechanical stimulus; negative regulation of endothelial cell proliferation; regulation of cGMP metabolic process; peptide cross-linking; response to progesterone stimulus; response to calcium ion; negative regulation of apoptosis; positive regulation of blood coagulation; positive regulation of translation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of caspase activity; behavioral response to pain; platelet degranulation; protein amino acid O-linked glycosylation; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of transforming growth factor-beta1 production; cell migration; chronic inflammatory response; negative regulation of focal adhesion formation; positive regulation of transforming growth factor beta receptor signaling pathway; engulfment of apoptotic cell; post-translational protein modification; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; cellular protein metabolic process; negative regulation of dendritic cell antigen processing and presentation; response to hypoxia; immune response; sprouting angiogenesis; blood coagulation; positive regulation of phosphorylation; positive regulation of cell migration