Tryptophan Hydroxylase 1 Recombinant Protein | TPH1 recombinant protein

Tryptophan Hydroxylase 1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121016

• Gene Names: TPH1; TPRH; TRPH
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Tryptophan Hydroxylase 1; Tryptophan Hydroxylase 1 Protein; Human; Recombinant (His Tag); Human Tryptophan Hydroxylase 1/TPH1 Protein (His Tag); TPRH Protein; TRPH Protein; tryptophan hydroxylase 1; TPH1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 200mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ile2-Ile444

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TPH1 (P17752-1)(Ile 2-Ile 444) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TPH1 recombinant protein

Background: Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme that belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.

Product Categories/Family for TPH1 recombinant protein

Neurotransmitter Associated Enzymes

References

Antonia S. New, et al.,1998, American Journal of Medical Genetics Part B. 81 (1): 13-17. Y. C Wang, et al.,2001, Neuropsychobiology. 43 (1): 1 - 4. Diego J. et al., 2003, Science. 299 (5603): 76. Allen NC, et al.,2008, Nat Genet. 40 (7): 827-34. Galfalvy,H. et al., 2009, J Affect Disord. 115 (3):331-8.

NCBI and Uniprot Product Information

• NCBI GI #: 4759248
• NCBI GeneID: 7166
• NCBI Accession #: NP_004170.1
• NCBI GenBank Nucleotide #: NM_004179.2
• UniProt Accession #: P17752; O95188; O95189; Q16736; Q3KPG8; D3DQX6
• Molecular Weight: 50,985 Da
• NCBI Official Full Name: tryptophan 5-hydroxylase 1
• NCBI Official Synonym Full Names: tryptophan hydroxylase 1
• NCBI Official Symbol: TPH1
• NCBI Official Synonym Symbols: TPRH; TRPH
• NCBI Protein Information: tryptophan 5-hydroxylase 1; L-tryptophan hydroxylase; tryptophan 5-monooxygenase 1; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)
• UniProt Protein Name: Tryptophan 5-hydroxylase 1
• Protein Family: Tryptophan 5-hydroxylase
• UniProt Gene Name: TPH1
• UniProt Synonym Gene Names: TPH; TPRH; TRPH
• UniProt Entry Name: TPH1_HUMAN

NCBI Description

This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]

Uniprot Description

TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.14.16.4

Chromosomal Location of Human Ortholog: 11p15.3-p14

Cellular Component: neuron projection; cytosol

Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding

Biological Process: circadian rhythm; aromatic amino acid family metabolic process; indolalkylamine biosynthetic process; negative regulation of ossification; positive regulation of fat cell differentiation; bone remodeling; serotonin biosynthetic process