TGFBR2 recombinant protein

Recombinant Rat TGFBR2 Protein (Fc tag)

Cat. Num. AAA2547453

• Gene Names: Tgfbr2; Tgfbr2T; TGF-beta 2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TGFBR2; Recombinant Rat TGFBR2 Protein (Fc tag); TGFBR2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 567

• Application Notes: The recombinant rat TGFBR2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 384 amino acids and has a predicted molecular mass of 43 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ile 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TGFBR2 recombinant protein

Background: TGFBR2 is member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised by a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilised by a network of six intra strand disulphide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various type s of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity.

Description: A DNA sequence encoding the rat TGFBR2 (P38438) (Met1-Gln166) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 94400786
• NCBI GeneID: 81810
• NCBI Accession #: NP_112394.3
• NCBI GenBank Nucleotide #: NM_031132.3
• UniProt Accession #: P38438
• Molecular Weight: 64,241 Da
• NCBI Official Full Name: TGF-beta receptor type-2
• NCBI Official Synonym Full Names: transforming growth factor, beta receptor 2
• NCBI Official Symbol: Tgfbr2
• NCBI Official Synonym Symbols: Tgfbr2T; TGF-beta 2
• NCBI Protein Information: TGF-beta receptor type-2
• UniProt Protein Name: TGF-beta receptor type-2
• Protein Family: TGF-beta receptor
• UniProt Gene Name: Tgfbr2
• UniProt Synonym Gene Names: TGFR-2; TGF-beta receptor type II; TbetaR-II

NCBI Description

receptor for TGF-beta; may play a role in lung maturation and response to hypoxia [RGD, Feb 2006]

Uniprot Description

TGFBR2: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Binds to DAXX. Defects can cause esophageal cancer.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type2 subfamily

Chromosomal Location of Human Ortholog: 8q32

Cellular Component: caveola; cell surface; cytoplasm; cytosol; external side of plasma membrane; integral to membrane; integral to plasma membrane; lipid raft; plasma membrane; receptor complex

Molecular Function: glycosaminoglycan binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor activity, type II; transmembrane receptor protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; aging; apoptosis; brain development; cartilage development; common-partner SMAD protein phosphorylation; embryo implantation; embryonic cranial skeleton morphogenesis; embryonic hemopoiesis; gastrulation; gut development; heart development; heart looping; in utero embryonic development; lens development in camera-type eye; lung development; myeloid dendritic cell differentiation; negative regulation of cardiac muscle cell proliferation; negative regulation of cell proliferation; Notch signaling pathway; organ morphogenesis; organ regeneration; palate development; patterning of blood vessels; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of angiogenesis; positive regulation of B cell tolerance induction; positive regulation of mesenchymal cell proliferation; positive regulation of NK T cell differentiation; positive regulation of skeletal muscle regeneration; positive regulation of smooth muscle cell proliferation; positive regulation of T cell tolerance induction; positive regulation of tolerance induction to self antigen; protein amino acid phosphorylation; receptor-mediated endocytosis; regulation of cell proliferation; regulation of gene expression; response to drug; response to estrogen stimulus; response to glucose stimulus; response to hypoxia; response to mechanical stimulus; response to nutrient; response to organic cyclic substance; response to organic substance; response to steroid hormone stimulus; smoothened signaling pathway; transforming growth factor beta receptor signaling pathway; vasculogenesis; wound healing