BAD Polyclonal Antibody | anti-BAD antibody

BAD (Phospho-Ser112)

Cat. Num. AAA191078

• Gene Names: Bad; Bbc2; AI325008
• Applications: Immunoblot, Western Blot, Immunohistochemistry
• Synonyms: BAD; Polyclonal Antibody; BAD (Phospho-Ser112); anti-BAD antibody

• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human and mouse BAD only when phosphorylated at serine 112.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 204

• Applicable Applications for anti-BAD antibody: Immunoblotting (IB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunoblotting: use at dilution of 1:500- 1:1,000. A ban of ~23kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from mouse BAD and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-BAD antibody

The protein encoded by the BAD gene is a member of the Bcl-2 family whose members are regulators of programmed cell death. BAD protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2 and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin, were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

NCBI and Uniprot Product Information

• NCBI GI #: 6671610
• NCBI GeneID: 12015
• NCBI Accession #: NP_031548.1
• NCBI GenBank Nucleotide #: NM_007522.3
• UniProt Accession #: Q61337
• Molecular Weight: 22,080 Da
• NCBI Official Full Name: bcl2-associated agonist of cell death isoform 1
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: Bad
• NCBI Official Synonym Symbols: Bbc2; AI325008
• NCBI Protein Information: bcl2-associated agonist of cell death
• UniProt Protein Name: Bcl2-associated agonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: Bad
• UniProt Synonym Gene Names: Bbc6; BAD

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 19|19 A

Cellular Component: cytoplasm; cytosol; mitochondrial outer membrane; mitochondrion

Molecular Function: caspase activator activity; lipid binding; phospholipid binding; protein binding; protein heterodimerization activity; protein kinase B binding; protein kinase binding; protein phosphatase 2B binding; protein phosphatase binding

Biological Process: ADP metabolic process; apoptosis; ATP metabolic process; caspase activation; cell proliferation; cellular process regulating host cell cycle in response to virus; cytokine and chemokine mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; glucose catabolic process; glucose homeostasis; induction of apoptosis via death domain receptors; negative regulation of cytolysis; pore complex biogenesis; positive regulation of apoptosis; positive regulation of apoptosis by virus; positive regulation of B cell differentiation; positive regulation of caspase activity; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of proteolysis; positive regulation of T cell differentiation; regulation of apoptosis; regulation of caspase activity; regulation of mitochondrial membrane permeability; release of cytochrome c from mitochondria; suppression by virus of host apoptosis