Rat Transforming Growth Factor Alpha ELISA Kit | TGFa elisa kit

Rat Transforming Growth Factor Alpha ELISA Kit

Cat. Num. AAA723748

• Gene Names: TGFA; TFGA
• Reactivity: Rat
• Synonyms: Transforming Growth Factor Alpha; Rat Transforming Growth Factor Alpha ELISA Kit; TGFa elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/ml
• Intended Uses: This TGFalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TGFalpha. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFa elisa kit

Principle of the Assay: TGFalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TGFalpha. Standards or samples are then added to the microtiter plate wells and TGFalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TGFalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TGFalpha are added to each well to "sandwich" the TGFalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TGFalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFalpha concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TGFa elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 339542
• NCBI GeneID: 7039
• Molecular Weight: 17,329 Da
• NCBI Official Full Name: transforming growth factor alpha, partial
• NCBI Official Synonym Full Names: transforming growth factor, alpha
• NCBI Official Symbol: TGFA
• NCBI Official Synonym Symbols: TFGA
• NCBI Protein Information: protransforming growth factor alpha; TGF-alpha
• UniProt Protein Name: Protransforming growth factor alpha
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFA
• UniProt Synonym Gene Names: TGF-alpha; ETGF
• UniProt Entry Name: TGFA_HUMAN

NCBI Description

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: extracellular space; cell surface; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to drug; cell proliferation; wound healing; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis