Human TGF alpha ELISA Kit | TGFA elisa kit

Human TGF alpha PicoKine ELISA Kit

Cat. Num. AAA175792

• Gene Names: TGFA; TFGA
• Reactivity: Human
• Synonyms: TGF alpha; Human TGF alpha PicoKine ELISA Kit; Protransforming growth factor alpha; transforming growth factor; alpha; EGF like TGF; ETGF; OTTHUMP00000203300; OTTHUMP00000203301; OTTHUMP00000203345; OTTHUMP00000203352; RATTGFAA; TFGA; TGF A; TGF type 1; TGFA; Transforming growth factor alpha; Transforming growth factor alpha precursor; Wa1; Waved 1; TGFA elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TGFalpha
• Sequence Length: 160

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and human milk
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human TGF alpha PicoKine ELISA Kit standard curve )

Related Product Information for TGFA elisa kit

Principle of the assay: human TGFalpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFalpha has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFalpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFalpha amount of sample captured in plate.
Background: Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain.1Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma.2 Synthetic TGF-alpha was as active as murine epidermal growth factor inbinding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.3

NCBI and Uniprot Product Information

• NCBI GI #: 135689
• NCBI GeneID: 7039
• UniProt Accession #: P01135; Q15577; Q53SK7; Q9BS56; Q9UEI3; Q9UKM1; Q9UKM2; Q9UKM3; A8K286
• Molecular Weight: 17,329 Da
• NCBI Official Full Name: Protransforming growth factor alpha
• NCBI Official Synonym Full Names: transforming growth factor, alpha
• NCBI Official Symbol: TGFA
• NCBI Official Synonym Symbols: TFGA
• NCBI Protein Information: protransforming growth factor alpha; TGF-alpha
• UniProt Protein Name: Protransforming growth factor alpha
• Protein Family: Transforming growth factor
• UniProt Gene Name: TGFA
• UniProt Synonym Gene Names: TGF-alpha; ETGF
• UniProt Entry Name: TGFA_HUMAN

NCBI Description

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: extracellular space; cell surface; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus

Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding

Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; wound healing; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis