Typical Testing Data/Standard Curve (for reference only)
General Triglyceride ELISA Kit | TGC elisa kit
General Triglyceride ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triglyceride, During the reaction, Triglyceride in the sample or standard competes with a fixed amount of biotin-labeled Triglyceride for sites on a pre-coated Monoclonal antibody specific to Triglyceride. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Triglyceride in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]
Uniprot Description
LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.
Protein type: Secreted; EC 3.1.1.3; Secreted, signal peptide; Phospholipase; Lipid Metabolism - glycerolipid
Chromosomal Location of Human Ortholog: 15q21-q23
Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space
Molecular Function: apolipoprotein binding; low-density lipoprotein binding; phospholipase activity; triacylglycerol lipase activity
Biological Process: cholesterol homeostasis; fatty acid biosynthetic process; lipid digestion; reverse cholesterol transport; triacylglycerol catabolic process
Disease: Diabetes Mellitus, Noninsulin-dependent; Hepatic Lipase Deficiency; High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12
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Product Notes
The General TGC lipc (Catalog #AAA2887844) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2887844 ELISA Kit recognizes General TGC. It is sometimes possible for the material contained within the vial of "Triglyceride, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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