Transferrin / TF Recombinant Protein | TF recombinant protein

Recombinant Rat Transferrin / TF Protein (His tag)

Cat. Num. AAA2547275

• Gene Names: Tf; Tfn; Trf
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Transferrin / TF; Recombinant Rat Transferrin / TF Protein (His tag); TF; TF recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.2
• Sequence Length: 698

• Application Notes: The recombinant rat TF comprises 690 amino acids and predicts a molecular mass of 76 kDa. The apparent molecular mass of the recombinant protein is approximately 89 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for TF recombinant protein

Background: Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins which bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe3+ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe (3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.

Description: A DNA sequence encoding the rat TF (NP_001013128.1) (Met1-Ser698) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 61556986
• NCBI GeneID: 24825
• NCBI Accession #: NP_001013128.1
• NCBI GenBank Nucleotide #: NM_001013110.1
• UniProt Accession #: P12346; Q63602; Q64628; Q64630; Q7TNX0
• Molecular Weight: 54,524 Da
• NCBI Official Full Name: serotransferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: Tf
• NCBI Official Synonym Symbols: Tfn; Trf
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• UniProt Gene Name: Tf
• UniProt Synonym Gene Names: Transferrin

NCBI Description

plays a role iin iron transport and homeostasis [RGD, Feb 2006]

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q32

Cellular Component: apical plasma membrane; basal plasma membrane; basement membrane; cell surface; coated pit; cytoplasmic vesicle; early endosome; endocytic vesicle; endosome; extracellular region; extracellular space; extrinsic to external side of plasma membrane; late endosome; membrane; perinuclear region of cytoplasm; recycling endosome; vesicle

Molecular Function: ferric iron binding; ferrous iron binding

Biological Process: actin filament organization; activation of JNK activity; acute-phase response; apoptosis; cellular iron ion homeostasis; ferric iron transport; iron ion transport; osteoclast differentiation; positive regulation of bone resorption; positive regulation of myelination; positive regulation of phosphorylation; positive regulation of receptor-mediated endocytosis; positive regulation of transcription, DNA-dependent; response to hypoxia; response to lead ion; response to organic cyclic substance; retinal homeostasis