Rat Gpx2 (Glutathione peroxidase 2) ELISA Kit | Gpx2 elisa kit

Rat Gpx2 (Glutathione peroxidase 2) ELISA Kit

Cat. Num. AAA7606152

• Gene Names: GPX2; GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
• Reactivity: Rat
• Synonyms: Gpx2 (Glutathione peroxidase 2); Rat Gpx2 (Glutathione peroxidase 2) ELISA Kit; Gpx2 elisa kit

• Reactivity: Rat
• Sequence Length: 190

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Gpx2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Gpx2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Gpx2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Gpx2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Gpx2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 32967607
• NCBI GeneID: 2877
• NCBI Accession #: NP_002074.2
• NCBI GenBank Nucleotide #: NM_002083.3
• UniProt Accession #: P18283; Q6PJ52; Q8WWI7; Q9NRP9
• Molecular Weight: 21,954 Da
• NCBI Official Full Name: glutathione peroxidase 2
• NCBI Official Synonym Full Names: glutathione peroxidase 2
• NCBI Official Symbol: GPX2
• NCBI Official Synonym Symbols: GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
• NCBI Protein Information: glutathione peroxidase 2
• UniProt Protein Name: Glutathione peroxidase 2
• Protein Family: Glutathione peroxidase
• UniProt Gene Name: GPX2
• UniProt Synonym Gene Names: GPx-2; GSHPx-2; GPx-GI; GSHPx-GI; GPRP-2

NCBI Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

Uniprot Description

GPX2: Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: cytoplasm; cytosol

Molecular Function: electron carrier activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species