Typical Testing Data/Standard Curve (for reference only)
Human Thymus Expressed Chemokine ELISA Kit | TECK elisa kit
Human Thymus Expressed Chemokine ELISA Kit
Gene Names
Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik
Reactivity
Human
Synonyms
Thymus Expressed Chemokine; Human Thymus Expressed Chemokine ELISA Kit; TECK elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TECK elisa kit
For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Uses: This TECK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TECK. This ELISA kit for research use only!
Principle of the Assay: TECK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TECK antibody and an TECK-HRP conjugate. The assay sample and buffer are incubated together with TECK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TECK concentration since TECK from samples and TECK-HRP conjugate compete for the anti-TECK antibody binding site. Since the number of sites is limited, as more sites are occupied by TECK from the sample, fewer sites are left to bind TECK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TECK concentration in each sample is interpolated from this standard curve.
Intended Uses: This TECK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TECK. This ELISA kit for research use only!
Principle of the Assay: TECK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TECK antibody and an TECK-HRP conjugate. The assay sample and buffer are incubated together with TECK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TECK concentration since TECK from samples and TECK-HRP conjugate compete for the anti-TECK antibody binding site. Since the number of sites is limited, as more sites are occupied by TECK from the sample, fewer sites are left to bind TECK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TECK concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TECK elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,733 Da
NCBI Official Full Name
thymus-expressed chemokine
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 25
NCBI Official Symbol
Ccl25
NCBI Official Synonym Symbols
TECK; CKb15; Scya25; AI852536; A130072A22Rik
NCBI Protein Information
C-C motif chemokine 25; thymus-expressed chemokine; small inducible chemokine 25; small inducible cytokine A25
UniProt Protein Name
C-C motif chemokine 25
UniProt Gene Name
Ccl25
UniProt Synonym Gene Names
Scya25; Teck
UniProt Entry Name
CCL25_MOUSE
Uniprot Description
CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Chemokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; cytokine activity
Biological Process: G-protein coupled receptor protein signaling pathway; immune response; chemotaxis; positive regulation of cell-matrix adhesion; inflammatory response; leukocyte migration
Research Articles on TECK
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Product Notes
The Human TECK ccl25 (Catalog #AAA727447) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727447 ELISA Kit recognizes Human TECK. It is sometimes possible for the material contained within the vial of "Thymus Expressed Chemokine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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